Light dependant reaction
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Where does the light dependant reaction take place?
In the thylakoids membrane.
Photosystem
Photosystems are large protein-pigment complexes with antennas in the thylakoid membrane. Each photosystem contains many chlorophyll molecules
What are the main two products we want from this reaction and why?
Reduced NADP and ATP as they are used for the light independent reaction
Photoionisation (First stage)
Photosystems antennas absorb light energy from the sun and transferred to reaction centre. Electrons in chlorophyll within reaction centre become excited and leave the molecule😀 Excited electrons passed through a series of electron carrier proteins embedded in the thylakoid membrane known as the electron transport chain
Production of ATP (chemiosmosis)
As electrons pass through the electron transfer chain through each protein they release energy. This is because they reduce the proteins when entering and oxidise them when leaving. This energy is used to pump H+ protons through the thylakoid membrane from the stroma to the thylakoid lumen by active transport. These protons return to the stroma by passing through the enzyme ATP synthase via facilitated diffusion (chemiosis - at every electron carrier electrons move to a LOWER energy level) ATP synthase then catalyses the reaction between ADP + Pi to form ATP. (Photophosphorylation)
Production of reduced NADP / NADPH (same thing lol)
Hydrogen ions in the stroma are combined with the co-enzyme NADP. ELECTRONS are also combined with NADP which is why it is reduced 2H+ + 2e- + NADP → NADPH
Photolysis
Light energy is also used to split water molecules in the thylakoids lumen. H2O → 2H+ + 2e- + ½O2
What are each of the products from photolysis used for
H+ protons are pumped across the thylakoid membrane during ATP production and they also join with NADP to form reduced NADP electrons are passed to the photosystem to replace those lost during photoionisation Oxygen is a waste product and diffuses out of the leaf cell
Exam tip
Photosystems is usually ignored by the mark scheme so use chlorophyll to be more specific