enzymes
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enzymes
make reactions in cells go at a speed necessary to maintain life
how do enzymes work
they speed up the rate of reactions by lowering the activation energy without being used up
substrate
is the molecules that is acted upon/ is changed by the enzyme
what is catecholase
an enzyme that is necessary in plants’ response to injury
what would happen without catecholase
enough benzoquinone would not be produced so plants’ tissue would stay damaged
cofactors
an inorganic molecules used to help enzymes carry out the reaction. Help achieve active conformation
what would happen without cofactors
enzymes active site would not be able to bind the substrate and the reaction wouldn’t catalyze
chelating agents
group of compounds that bind metallic ions and by removing the ions, change the shape of the enzyme
why is the potato chilled
to stunt any benzoquinone production
why is half a potato used
a whole potato contains too many enzymes
why is the potato peeled
because the brown peel of the potato could affect the absorbance value interpreted by the spectrophotometer
why is distilled water added to the blender
to ensure a hypotonic environment
what absorbance value is the spectrophotometer set to
its set to 540nm
why is the spectrophotometer set to 540nm
benzoquinone is a reddish-brown color, the opposite of red on the color wheel is green. green is the color that is absorbed maximally by benzoquinone