pH Scale And Standard Curve
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How is the concentration of hydrogen ions determined?
By measuring the current generated in an electrochemical cell when the ions migrate to oppositely charged electrodes
What is a buffer solution?
a solution that changes pH only slightly when small amounts of a strong acid or a strong base are added
What does a buffer contain
A weak acid with its salt (conjugate base) OR A weak base with its salt (conjugate acid)
How does a buffer solution work? 2
The acid component of the buffer can neutralize small added amounts of OH-, The basic component can neutralize small added amount of H3O+
What is an assay?
An assay is a method used to measure something
What is the biuret method of preparing an assay? 2
The blue reagent (biuret reagent) turns violet in the presence of proteins, and changes to pink when combined with short-chain polypeptides Peptide-bond react with alkaline copper-sulfate to produce a blue/violet colour
Define a response curve.
A response curve is a plot of the assay responses versus a series of known analyte concentrations
Define a standard curve
Linear portions of response curves are called standard curves
Define Beer’s Law
The relationship between absorbance and the concentration of a compound is linear except at very low and high concentration and in impure solutions