Final Practical
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Renal threshold Dog/Cat
160-180 mg/dl 280-300 mg/dl
Normal blood glucose for dogs
100mg/dl
How long is a urine sample good for in the refrigerator/room temp
6hrs. refrig 30min. room temp
4 methods of collecting a urine sample
Voided/free catch Manual expression Catheterization Cystocentesis
What happens to urine if left out too long?
Bacteria multiplies Crystals increase Urine becomes cloudy Cells disintegrate pH changes
How to preserve urine
1gtt of 40% formaldehyde to 1 oz. urine
4 parts to a routine U/A
Macroscopic USG Urine chemistries Microscopic
Fixed USG range
1.008-1.012 2/3 - 3/4 of the nephrons are non-functional
Normal BUN
7-27mg/dl
Normal USG in dog
1.018-1.045
Normal creatinine in Dog/Cat
• 5-1.8mg/dl 0. 8-2.4mg/dl
Centrifuge urine at ____rpm for ___ min. Pour off ____ resuspend the ___ scan on ___ and do final analysis on __
1500, 3min supernatent sediment 10X, 40X
Sulfosalicylic Acid Test
confirms the presence of protein in urine
Blood spun at ____ for ____
6000rpm for 5min
Why is serum the preferred sample for biochemical profiling, and what is the exception?
To avoid anticoag. interference lithium heprin plasma
Tube used for effusions- bacterial & cytology
Bacterial- RTT Cytology- LTT
Griffin beaker
Come in all sizes Graduated NOT accurate for measuring used for holding and mixing
Erlenmeyer Flask
Narrow neck and flared bottom Graduated NOT accurate for measuring used for holding and mixing
Volumetric Flask
Long neck and round bottom Measures one volume only
Graduated cylinder
Different sizes Graduated Choose smallest that will hold Used for measuring
Pasture Pipette
Glass pipette with a bulb Disposable Remove serum or plasma from tube of blood NOT for measuring
Plastic transfer Pipette
plastic pipette disposable NOT used for measuring
Serological Pipette
Glass Graduated Use with bulb or pump Measures volume
Volumetric Pipette TC TD
Glass Bulge in the middle Measures one volume only Most accurate for measuring volume TC= To Contain- blow out TD= To Deliver