Potassium
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activated by K+ ions in the sample and subsequently catalizes the dephosphorylation of phosphoenol- pyruvate to pyruvate.
Pyruvate kinase
In a second step pyruvate is transformed to
Lactate under consumption of a NADH analogue
The rate of the signal decrease measured at ______ is proportional to the amount of potassium in the sample.
340 nm
R1:
Buffer NADH analogon Phosphoenolpyruvate ADP Lactate dehydrogenase
R2:
Buffer Pyruvate kinase
Specimen
Serum or lithium heparin plasma
Interfering Substance
Ascorbate Bilirubin, conjugated Bilirubin, unconjugated Lipemia Hemoglobin Hemolysis Sodium Ammonia Calcium Magnesium Manganese Phosphate Zinc Iron Copper
Conversion factor
3.91 mg/dL
Reference Range
In Plasma: 3.6 – 4.8 mmol/L In Serum: 3.5 – 5.1 mmol/L