lecture 14

Differences or changes in function or outcome

Changes in gene sequence

Mutations can be made

endonucleases that recognize specific DNA sequences and cleave DNA

Type 2 Restriction Enzymes recognize and cut within a…

Type 2 Restriction Enzymes activity doesnt require energy (ATP) but needs…

Type 2 Restriction Enzymes sites are usually ____ 4-8 bp long

Type 2 Restriction Enzymes hydrolysis of phosphodiester bonds in the sugar-phosphate backbone of both strands of DNA

Type 2 Restriction Enzymes Cuts result in ____ or ____ (5’ or 3’ overhangs)

Type 2 Restriction Enzymes Activity can be prevented by…

Type 2 Restriction Enzymes Preventing activity Allows degradation of foreign DNA while leaving host DNA

Seperates cgarged molecules migrating in an electrical field. Charge, size, and shape dtermine mobility

Electrophoresis in a gel such as the polysaccharide ____ seperates linear DNA fragments by ____

Often hybridization is done using ____ DNA fragments called primers or ____

Making many identical copies of somethimg

Making many identical copies of a molecule-typically a fragment of DNA of interest

Molecular cloning is usaully done by moving a fragment of DNA into a ____ called a cloning ____ which is moved into a suitable host

Molecular cloning Manipulation often done using restriction ____ and DNA ____

Engineered DNA fragments are called recombinant meaning

Cloning with pUC 19 Digest vector and DNA insert with appropriate ____ ____

Cloning with pUC 19 Join with DNA ____

Cloning with pUC 19 Introduce into appropriate host plate on solid medium containing ____ and ____

Product of gene lacZ is

B-gal can cleave the substrate ____, resulting in a ____ colored product