Carbohydrates 2

the standard clinical specimen.

Fasting glucose in whole blood is _ lower than in serum/plasma.

perform glycolysis using energy from serum/plasma biomolecules.

suitable for glucose analysis if separated from cells within 30 minutes (via centrifugation).

If delayed >30 minutes, use glycolysis-inhibiting preservatives like

Venous blood glucose is _ lower than the capillary due to tissue metabolism (extrahepatic tissues such as in the muscles)

NOTE: _ is the same with arterial blood glucose

Plasma glucose level increases with age: ○ Fasting - _/dL /decade ○ Postprandial - _/dL /decade ○ Glucose challenge test – _ mg/dL /decade

Specimen Handling and Storage Prolonged storage: at room temperature between _ _ _, glycolysis decreases glucose by 7 mg/dL/hr in normal uncentrifuged blood

Specimen Handling and Storage At refrigerated temperature of _ , glucose is metabolized at the rate of 2 mg/dL/hr

the suitable specimen for glucose testing because → WBC and RBC metabolize glucose resulting to decrease value in clotted, uncentrifuged blood

Principle: reduction of cupric ions to cuprous ions forming cuprous oxide in hot alkaline solution by glucose

Glucose is being subjected to

● End point / color reaction: _ ● Measured in optical density: _

Reducing sugars, when heated in alkaline copper tartrate, reduce copper to cuprous or cupric form

Cuprous oxide reacts with arsenomolybdic acid, forming a blue color

Nelson Somogyi Method Color intensity is measured at

Copper reacts with neocuproine to form the cuprous-neocuproine complex

The complex is extracted from the chloroform of methanol mixture yielding a yellow or yellow-orange solution ● End point / color reaction: _

It is used for the detection and quantification of reducing substances in body fluids (blood and urine)

standard clinical specimen

Fasting glucose in whole blood is _ lower than in serum/plasma

_ perform glycolysis using energy from serum/plasma biomolecules.

suitable for glucose analysis if separated from cells within 30 minutes (via centrifugation).