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Method for Glucose Determination: -Based on the phosphorylation of glucose to produce 6-phosphogluconate -most specific enzymatic method

Method for Glucose Determination: Based on the oxidation of B-D- glucose to D- gluconic- lactone and hydrogen peroxide

INDICATORS OF GLUCOSE OXIDASE: Aminoantipyrine + Phenol quinone imine dye complex formation is measured at 510-540 nm Commonly used in Dry Chemistry Red Product

Electrochemical detection Electrode measures oxygen consumption

Electrochemical detection Electrode measures oxygen consumption

Measures the rate of oxygen consumption Rate of consumption= concentration of glucose It can be utilized for serum, urine, and spinal fluid but not in whole blood sample

The amount of NADH generated is proportional to the glucose concentration

Indicator: POCT: GDH

POCT: GDH yellow

POCT: GDH Colorless

CHEMICAL METHODS: Reduction of a yellow ferricyanide to a colorless ferocyanide by glucose Inverse Colorimetry

Chemical Method: Condensation Method Most Sensitive

ortho-toluidine: sample is mixed with ___________ acid and then heated

Chemical Method: based on the reduction of Cu2+ ions to Cu+ ions in the presence of reducing sugars.

Chemical Method: based on the reduction of Cu2+ ions to Cu+ ions in the presence of reducing sugars.

Cu+ ions further reduced the ________________ complex

Reduction of arsenomolibdate complex produced a stable ______-colored dye and can be measured spectrophotometrically at ______nm

The arsenomolibdate complex is prepared by reacting ____________ and _____________ in sulfuric acid

based on precipitation of proteins by tungstic acid and reduction of the alkaline copper solution by blood sugar

The amount of ___________ ________ formed being estimated colorimetrically by reacting with _________________ ________

The cuprous is oxidized to cupric and the phosphomolybdic acid reduced to ______________