LIVER FUNCTION TEST part 4

uses Diazotized sulfanilic acid for Bilirubin used in serum

Van Den Berg Reaction uses this acid in serum.

the diazotized sulfanilic acid is formed by reacting sulfanilic acid with _________________

This is often subjective and the test involves diluting serum with saline until it visually matches the color of a 0.01% potassium dichromate solution

Icterus Index test involves diluting serum with __________ until it visually matches the color of a ____________________

What are the interferences in Icterus Index?

In DIRECT SPECTROPHOTOMETRIC METHOD, what is the wavelength at which bilirubin in serum is measured?

the absorbance of hemoglobin is 455 nm which can be corrected by subtracting the absorbance at ____________

Problem causing jaundice occurs before liver metabolism

What caused the pre-hepatic Jaundice?

Unconjugated hyperbilirubinemia

Intrinsic liver disease

In Gilbert’s Syndrome, this is reduced in the expression of gene.

 intermittent hyperbilirubinemia  inherited bilirubin derangement  Reduced conjugating capability of the liver

 has a defective hepatic uptake of bilirubin

has a defective conjugation of bilirubin due to the deficiency of the enzyme UDPGT

• Chronic unconjugated hyperbilirubinemia • Molecular defect w/in the gene involved with bilirubin conjugation • Rare & more serious disorde

 total UDPGT deficiency  complete absence of enzymatic bilirubin conjugation

 partial UDPGT deficiency  the mutation causing a severe deficiency of the enzyme for Conjugation

defective hepatic excretion of bilirubin

What caused the Dubin-Johnson Syndrome?

defective hepatic excretion of bilirubin; has normal gall bladder function

What causes the Rotor Syndrome?

TRUE OR FALSE: Liver biopsy does NOT show dark pigmented granules