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[LAB] AST & ALT

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Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (24)

#1
Front (Question)

EC CODE OF AST

Back (Answer)

2.6.1.1

#2
Front (Question)

EC CODE OF ALT

Back (Answer)

2.6.1.2

#3
Front (Question)

NORMAL VALUES OF AST

Back (Answer)

30C — Up to 28 37C — Up to 40 QUICK: 28–30 40–37

#4
Front (Question)

MULTIPLIER OF AST and ALT

Back (Answer)

x 1768

#5
Front (Question)

SI CONVERSION FACTOR OF AST AND ALT

Back (Answer)

x 16.67

#6
Front (Question)

CONC. OF 2-OXOGLUTARATE IN AST REAGENT

Back (Answer)

0.12 mM

#7
Front (Question)

CONC. OF 2-KETOGLUTARATE IN ALT REAGENT

Back (Answer)

0.13 mM

#8
Front (Question)

CONC. OF L-ASPARTATE IN AST REAGENT

Back (Answer)

200 mM

#9
Front (Question)

CONC. OF L-ALANINE IN ALT REAGENT

Back (Answer)

400 mM

#10
Front (Question)

CONC. OF LDH AND MDH IN AST REAGENT

Back (Answer)

LDH — 800 mM MDH — 600 mM

#11
Front (Question)

DETECTION LIMIT/ LINEARITY OF AST AND ALT

Back (Answer)

Up to 500 IU/L

#12
Front (Question)

TROUBLESHOOTING SAMPLES WITH VALUES HIGHER THAN THE LINEARITY

Back (Answer)

• Dilute with 1:1 Saline Solution • Re-assay • Multiply results by 2

#13
Front (Question)

STORAGE CONDITION OF DRY REAGENTS OF AST AND ALT

Back (Answer)

Store at 2-8C

#14
Front (Question)

STABILITY OF RECONSTITUTED REAGENTS

Back (Answer)

Stable for 8 hours at RT (15-30C) OR 21 days when refrigerated immediately

#15
Front (Question)

STABILITY OF SPECIMEN

Back (Answer)

Stable at 4C for a minimum of 7 days

#16
Front (Question)

ALT ACTIVITY IN HEMOLYZED SERUM

Back (Answer)

7x ALT ACTIVITY

#17
Front (Question)

AST ACTIVITY IN HEMOLYZED SERUM

Back (Answer)

15x AST ACTIVITY

#18
Front (Question)

NORMAL VALUES OF ALT

Back (Answer)

30C — Up to 26 IU/L 37C — Up to 38 IU/L QUICK 26 — 30 38 — 37

#19
Front (Question)

RECONSTITUTE DRY REAGENT WITH __ mL of ___

Back (Answer)

12 mL of Distilled Water

#20
Front (Question)

WAVELENGTH OF AST AND ALT ASSAYS

Back (Answer)

340 nm

#21
Front (Question)

BLANK SOLUTION COMPOSITION

Back (Answer)

1.0 mL of Distilled Water

#22
Front (Question)

SAMPLE SOLUTION COMPOSITION

Back (Answer)

1.0 mL Reagent 0.1 mL Patient’s Sample

#23
Front (Question)

AST/ALT INCUBATION TEMPERATURE AND PERIOD

Back (Answer)

37C for 3 minutes

#24
Front (Question)

AST/ALT INCUBATION TEMPERATURE AND PERIOD IN BETWEEN READINGS

Back (Answer)

37C for 1 minute