Drug Testing
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What type of immunoassay is drug testing?
Rapid Qualitative Competitive immunoassay
Other methods fro drug testing
Gas chromatography/ Mass Spectrophotometry
Drugs that can detect in drug testing
THC (Marijuana) METHAMPHETAMINE
THC (MARIJUANA) is derived from
Flowering portion of the hemp plant
Cannabinoids has been proposed for therapy
Acute glaucoma and parsea due to chemotherapy
Higher used of marijuana can cause
CNS effect Altered mood Sensory preceptions Loss of coordination Impaired shortterm memory Snxiety Paranoia Depression Confusion Hallucinations Increast heart rate
Withdrawal syndrome of marijuana can cause
Restlesness Insomia Anorexia Nausea
Where does marijuana metabolizes
Liver
The primary urinary metabolite
11-nor-🔺-9- tetrahydricannabinol-9 carboxylic acid and its glucorinade
Methamphetamine acute higher dose lead to enhancement
CNS induced euphoria Alertness Sense of increased energy and power
Cutoff level of cannabinoid
50 ng/ml in urine
Methamphetamine screening cutoff
500 ng/ml
Prinicple of drug testing
The Advanced Quality One Step Multi-Drug Screen Test card is a device composed of 2-6 chromatographic strips designed to detect 2-6 (as per the format) individual drugs of abuse. Each strip consists of a sample pad treated with antibody colloidal gold conjugate and membrane treated with drug conjugate and control reagent. Urine sample initially reacts with the antibody gold conjugate, and then migrates up the strip, by capillary action, to the test area. If sufficient drug is present in the urine, it binds with the conjugate, preventing it from binding to the drug conjugate immobilized on the membrane in the test region. Any unbound conjugate continues to migrate up the strip to the control region where it binds to the control reagent producing a pink/purple band. The control band indicates that the result is valid.
WARNINGS AND PRECAUTIONS
• For in vitro diagnostic use only. 2.For professional Medical and Forensic use only. 3.Do not use the kit beyond the expiration date imprinted on the outside of the foil pouch. • Do not open the foil pouch until the urine is collected and ready to be tested. 5.Avoid cross contamination of urine samples by using a new urine sample cup for each sample. 6.Urine specimens may be infectious. Upon completion of all testing dispose of residual urine in an approved manner. Properly handle anddispose of all used reaction devices in a biohazard container.
Procedure
1.Bring all materials and specimens to room temperature. 2.Remove test device from the sealed foil pouch. 3.Remove the cap from the device. 4. Immerse the bottom end of the test strips (the sample pads) into urine sample with the arrows pointing toward the specimen. (keep the level of urine sample below the bottom of plastic card or the maximum line marked on the strips.) 5.Hold the device in the urine until a reddish color appears at the lower edge of the test membrane (approximately 10 seconds). 6. Withdraw the device from urine sample, and replace the cover. 7.Read results between 3 - 8 minutes.