enz 1 pt 2
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transition state 5
high energy conformation most stable state lowest enz energy, highest subs energy enz releases a lotttt of E to stabilize the sys. ENZ DOSES NOT DECREASE THE ACTIVATION ENERGY OF THE RXN the TS gives time for the chem rxn to occur
negative free energy (delta G)
rxn is spontaneous / favorable for a + b –> c + d sys is STABLE
positive free energy (delta G)
reaction is NON-spontaneous / favorable for a + b –> c + d
zero free energy (delta G)
the rxn is at eq. No gain or loss of E
does the rate of forward rxn = to the rate of reverse rxn in a cell>?
yes
high E bonds 4
when the products are more stable/abundant than the reactants due to: - more resonance forms, - same electrostatic qs in proximity, - isomerization potential (molecule is transformed into an isomer w/ a diff chem structure)
Vmax
theoretical value of max velocity of an enz. NEVER achieved
Km 3
substrate conc. that results in Vmax / 2 represents [S] where 1/2 of the active sites of enz are filled + tells us the strength of the ES complex. Km chamge = enz changes due to Vmax changes
Plateu curve
when the rate of the reaction starts to decrease and approach Vmax
velocity (v) equation or Michaelis-Menten Equation
v = vmax * [S] // [S] + Km
Km is a small number
meaning it takes LOWER levels of [S] to fill the active site and the ES (binding activity) complex is STRONG enz finds subs more successfully.
Km is a large number
meaning it takes HIGHER levels of [S] to fill the active site and the ES (binding activity) complex is WEAK enz has a harder time to find subs