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experimental approaches

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Total cards: 12•Content version: v3•Public deck•Updated: 8/1/2026

Card preview (12)

#1
Front (Question)

what does hybridization refer to

Back (Answer)

the association via complementary base-pairing of two single stranded nucleic acids to form a double stranded molecule

#2
Front (Question)

list 3 types of hybrids produced from hybridization

Back (Answer)

DNA-DNA, RNA-RNA, DNA-RNA

#3
Front (Question)

what is southern blotting used to detect

Back (Answer)

fragments of a specific sequence

#4
Front (Question)

list the steps of southern blotting

Back (Answer)

digest genomic DNA, run on gel to separate, denature the fragments via alkali solution, blot fragments onto membrane via capillary action, incubate with the probe = hybridization step, identify the fragments on the membrane

#5
Front (Question)

list 3 ways that the fragments of southern blotting can be identified

Back (Answer)

autoradiography, chemiluminescnece, fluorescent imaging

#6
Front (Question)

what does northern blotting detect

Back (Answer)

RNA

#7
Front (Question)

where are riboswitches found

Back (Answer)

5’ UTR of mRNA

#8
Front (Question)

not a flashcard, but go review the glycine riboswitch section for S pyogenes

Back (Answer)

you’re slaying :)

#9
Front (Question)

what is western blot used to detect

Back (Answer)

protein

#10
Front (Question)

list the steps of a western blot

Back (Answer)

isolate protein, separate using SDS-PAGE, transfer proteins from gel to membrane, detect protein of interest

#11
Front (Question)

what does SDS-PAGE stand for

Back (Answer)

sodium dodecyl sulfide polyacrylamide gel electrophoresis

#12
Front (Question)

list 3 modes of detection of the protein from a western blot

Back (Answer)

colorimetric, chemiluminescence, fluorescence