CC2 LAB: LIPASE AND AMYLASE
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to catalyze oxidation/reduction reactions
oxidoreductases
to transfer functional group from one substance to another
transferases
formation of 2 products from a substrate by hydrolysis using water
hydrolases
intramolecular rearrangements
isomerases
non-hydrolytic addition or removal of groups from substrates
lyases
join together 2 molecules by the synthesis of new bonds
ligases
lipase is from what major class of enzymes
hydrolases
systematic name of lipase
triacylglycerol acylhydrolase
specific enzymes inside the pancreas
lipase amylase
substrate of lipase
fats
products of lipase
alcohol fatty acids
activators of lipase
colipase bile salts
what does lipase attacks?
ester linkages
classic-cherry crandall method modified substrate
triolein
lipase acute pancreatitis: onset
4-8 hours
lipase acute pancreatitis: normalize
8-14 days
lipase acute pancreatitis: peak
24 hours
lipase assay optimal ph
8.8 pH
lipase assay normal temp
40 degrees celsius
what methods does lipase assay do
titration turbidimetric method
classic-cherry crandall method substrate
olive oil
what does the method classic-cherry crandall used
titration
in lipase what enzymes are based on coupled reactions
peroxidase glycerol kinase
in what sample or specimen is the lipase stable
serum