Cell Respiration
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Why did cell resp evolve
What are the pos and neg feedback loops
Usually have to do with the amount of ATP, if there is a lot of ATP then it will bind to some of the enzymes and inhibit them if there not a lot of ADP then it will cause the enzymes to increase production
Which steps in cell resp can be shut down and which ones continue regardless
Glycolysis will continue regardless the others will shut down
When and whyuse fermentation
when pyruvate accepts the electron from NADH made in glycolysis instead of O2 Allows the glucose to still be processed and make ATP when there not enough final electron receptor(Oxygen) for the for the electron transport chains converts Nadh back into Nad+ only makes 2 ATP, very inefficient
What are the 2 types of fermenation
lactic acid fermentation - pyr accepts elect from NADH, makes NAD+ and lactate. NAD+ is used again in glyc For mammals, more common alcohol fermenation - pyr is converted into acetaldehyde and CO2 Acetaldehyde accepts elect from NADH Ethanol and NAD+ are produced for orgs like yeast, unicellular moulds NAD+ is oxidized in fermentation
What are the 4 main steps of cell resp
glycolysis - gluc broken down into pyr pyruvate oxidation - pyr processed to make acetyl-Coa krebs cycle - Acetyl-Coa is processed to CO2 electron transport chain - has oxidative phosphorylation
How does the location change througout cell resp
Glycolysis into pyruvate happens in cytosol pyr goes into mitochondria, through its 2 memb, into cytosol of mitochondria krebs happens in the cytosol of mitochondria etc happens in the inner memb of mito
make analogy of atp and NADH in cell resp
like money, atp is the base currency NADH is another currency that needs ot be converted into ATP to be of use. same with FADH2
what is oxidation of glucose vs oxidative phosphorylation
the first 3 steps in cell resp are ox of glucose (glycolysis, pyr ox, krebs) etc + atp synthase is oxidative phosphorylation
Why do things use starch to store sugars, why is this important to use
It is insoluble in water storage capacity for glucose doesn’t degrade in the presence of water which is really important humans eat the foods like potatos that use starch to store sugars.
Summary of glycolysis
10 chem reactions makes 2, 3 carbon molec called pyruvate NAD+ is reduced to NADH has net 2 ATP (uses up 2, makes 4) has net 2 NADH regulated with neg feedback loo
How is glycolysis regualted
with neg feedback loop high levels of ATP inhibit enz used in glyc ATP can also bind to the active site but if there is high conct of ATP some will bind to regulatory site and slow it down If you have too much ATP then you don’t need to run the second half of glyc, enz is in step 4
Summary of pyruvate ox
In the presence of ox it becomes Acetyl-coa Acetyl Coa is 2 carbon molec, pyr is 3 One of the carbon atoms from pyr is lost as CO2 Takes place in mitochondrial matrix, and then Acetyl-Coa goes into mitochondria Makes CO2 an NADH has Pos and Neg feedback
Where does pyr ox happen in bact and archaea
If the bact or arch is just the mitochondria then it would just happen on the inside of the bact Happnes in the cytosol of the bacteria and archaea
How is pyr ox positive and negative feedback
if there is high ATP levels then it does negative feedback also if there is not a lot of NAD+ it slows down so that it doesnt get depleted NADH is used as positive feedback
Summary of krebs
Third and final step in oxidation of glucose Acetyl Coa is oxidiced into 2 CO2 8 molecs are used but none of them are used up makes 3NADH and1 FADH2 and 1ATP happens in mitochondrial matrix has neg feedback loop, regulated by ATP and NADH levels
Summary of electron chain transport
electrons in NADH and FADH2 are decreased in redox reactions uses protiens in the innber memb of mitochondria, makes up etc etc used to pump protons accros memb, makeing electrochem gradiant electrons end up at O2 Uses proton motive force to drive atp synthase
How is proton motive force generated
protein complexes in the mito memb reduce the NADH and FADH and push the protons into the intermemb space keeps conct high so that they are trying to get in through the atp synth