Back to marketplace

ALP

No description yet. This curated deck is ready for daily study.

Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (24)

#1
Front (Question)

What is the known function of ALP?

Back (Answer)

Lipid transfer in the intestine and bone formation

#2
Front (Question)

This hydrolyzes naturally occurring substrate.

Back (Answer)

ALP

#3
Front (Question)

What is the optimum pH of ALP?

Back (Answer)

10.3

#4
Front (Question)

What is the systematic name of ALP?

Back (Answer)

E.C. 3.1.3.1

#5
Front (Question)

What are the major isoenzymes of ALP?

Back (Answer)

• Placental Isoenzyme • Intestinal Isoenzyme • Liver Isoenzyme • Bone Isoenzyme

#6
Front (Question)

What method is used for ALP measurement?

Back (Answer)

Bowers and McComb

#7
Front (Question)

This method uses p-NPP substrate. Thus, chelators falsely lower activity. First, enzyme activity increases slightly on storage and is relatively stable at 4’C up to a week.

Back (Answer)

Bowers and McComb

#8
Front (Question)

In storage, at what rate does enzyme activity increase per day?

Back (Answer)

2%

#9
Front (Question)

Under Bowers and McComb, enzyme activity will remain stable at what temperature?

Back (Answer)

4’C up to a week.

#10
Front (Question)

If the sample is frozen. What should we do?

Back (Answer)

• Thaw • Store the sample at RT for 18 - 24hrs before testing.

#11
Front (Question)

If the sample is frozen. What should we do?

Back (Answer)

• Thaw • Store the sample at RT for 18 - 24hrs before testing.

#12
Front (Question)

ALP reaction only takes place in the presence of cofactors (disulfide ions). What are these?

Back (Answer)

• Cobalt • Magnesium • Manganese • Zinc

#13
Front (Question)

This cofactor serves as a constituent metal ions and must combine with ALP reaction to take place because without this factor ALP reaction would be incomplete.

Back (Answer)

Zinc

#14
Front (Question)

What are the Inhibitors of ALP?

Back (Answer)

• Phosphates • Borate • Oxalate • Cyanide ions

#15
Front (Question)

If the anticoagulant contains these inhibitors, it should never be used in measuring ALP. What are these?

Back (Answer)

Borate and Oxalate

#16
Front (Question)

What isoenzymes do this inhibit? A. Phenylalanine B. Levamisole

Back (Answer)

A. Intestinal and Placental B. Liver and Bone

#17
Front (Question)

This separation of enzymes is differentiated based on heat stability.

Back (Answer)

Heat fractionation.

#18
Front (Question)

In heat fractionation, at what degrees is the enzyme exposed to?

Back (Answer)

56’C for 10 minutes

#19
Front (Question)

What isoenzyme is separated in Heat Fractionation?

Back (Answer)

All except Intestinal Isoenzyme

#20
Front (Question)

In electrophoresis, it is exposed to what enzyme?

Back (Answer)

Neuraminidase

#21
Front (Question)

In electrophoresis, what are we going to do with the neuraminidase?

Back (Answer)

For 15 minutes, pretreat the neuraminidase + serum @ 37’C

#22
Front (Question)

What are the substrates used in Electrophoresis?

Back (Answer)

• Agarose gel • Polyacrylamide gel • Cellulose acetate

#23
Front (Question)

This improves separation of bone and liver form. Differences in their CHO side chains.

Back (Answer)

Use of enzymes or lectins

#24
Front (Question)

What overlaps with bone isoenzyme?

Back (Answer)

Placental Isoenzyme