ORGANIC CHEM - CHROMATOGRAPHY
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What is the mobile phase?
the substance that moves (solvent/inert gas)
What is the stationary phase?
the thing that the substances travel up (doesn’t move) (paper/powder/liquid)
What are the 4 types of chromatography?
• paper • thin layer chromatography (TLC) • column • gas (/gas-liquid)
What is the stationary phase in paper chromatography?
chromatography paper
What is the stationary phase in thin layer chromatography?
powder (SiO2/Al2O3) on plastic sheet
What is the stationary phase in column chromatography?
powder (SiO2/Al2O3)
What is the stationary phase in gas (/gas-liquid) chromatography?
powder packed in tube/liquid coated on tube lining
What is the mobile phase in paper chromatography?
solvent
What is the mobile phase in thin layer chromatography?
solvent
What is the mobile phase in column chromatography?
solvent
What is the mobile phase in gas (/gas-liquid) chromatography?
inert gas (eg: He/Ar/N2)
Why is a lid necessary in paper chromatography/TLC?
• prevents solvent from evaporating • ensures atmosphere is saturated with solvent
Why should gloves be used when holding a TLC plate?
to avoid contamination
How can colourless samples be seen?
UV light
How can amino acids be made visible?
stain with ninhydrin