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Liver Function (Bilirubin Determination)

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Total cards: 20•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (20)

#1
Front (Question)

Bilirubin determination specimen

Back (Answer)

Fasting Serum

#2
Front (Question)

Bilirubin Determination Specimen Requirements

Back (Answer)

Avoid Exposure to light Avoid Hemolysis (false decreased) Lipemia (falsely increased)

#3
Front (Question)

Exposure to light; decreases bilirubin ____ per hour

Back (Answer)

30-50%

#4
Front (Question)

Bilirubin Determination Principle

Back (Answer)

Van De Berg Reaction

#5
Front (Question)

diazotization of bilirubin to produce AZOBILIRUBIN

Back (Answer)

Van De Berg Reaction

#6
Front (Question)

Diazo Reagent consist of (2)

Back (Answer)

0.1% Sulfanilic acid + HCI 0.5% Sodium Nitrite

#7
Front (Question)

Reacts with diazo reagent with an accelerator

Back (Answer)

B1

#8
Front (Question)

Reacts with diazo reagent without accelerator

Back (Answer)

B2

#9
Front (Question)

Reaction with diazo reagent is same with B2

Back (Answer)

Delta Bilirubin

#10
Front (Question)

No coupling accelerator; determine DIRECT BILIRUBIN ONLY

Back (Answer)

Direct Method

#11
Front (Question)

With Accelerator; Determines both B1 and B2

Back (Answer)

Indirect Method

#12
Front (Question)

Indirect Method (2)

Back (Answer)

Evelyn Malloy Jendrassik-Grof

#13
Front (Question)

Accelerator: 50% Methanol Stopper: None pH: acidic (1.2) End color: Red-Purple Absorbance: 560 nm

Back (Answer)

Evelyn Malloy

#14
Front (Question)

Accelerator: Caffeine benzoate Stopper: Ascorbic acid pH: Alkaline Tartrate/Sodium Acetate Buffer End color: PINK-BLUE Absorbance: 600 nm

Back (Answer)

Jendrassik-Grof

#15
Front (Question)

Determine Bilirubin concentration by measuring at 455 nm This method is only used for measuring total Bilirubin HDN

Back (Answer)

Direct Spectrophotometry

#16
Front (Question)

Bilirubin is oxidized to biliverdin ; decreased absorbance at 405 nm - 460 nm Not affected by Hemoglobin

Back (Answer)

Bilirubin Oxidase Method

#17
Front (Question)

Sensitive marker for impaired hepatic sythetic function

Back (Answer)

Prothrombin Time

#18
Front (Question)

Increase Polyclonal IgG

Back (Answer)

Autoimmune Hepatitis

#19
Front (Question)

Increase Polyclonal IgM

Back (Answer)

Primary Biliary Cirrhosis

#20
Front (Question)

A/G ratio in Liver disease is <1.0

Back (Answer)

Gamma Globulins