AST
No description yet. This curated deck is ready for daily study.
Card preview (17)
Components and Concentrations: R1
TRIS (pH 7.65) - 110 mmol/L L-Aspartate - 320 mmol/L MDH (malate dehydrogenase) - >_ 800 U/L LDH (lactate dehydrogenase) - >_ 1200 U/L
Components and concentrations R2
2-Oxoglutarate - 85 mmol/L NADH - 1 mmol/L
Components and Concentrations: Pyridoxal-5-phosphate
Good’s buffer (pH 9.6) - 100 mmol/L Pyridoxal-5-phosphate - 13 mmol/L
Warnings and precautions: Reagent 1
Contains animal and biological material
Warnings and precautions: Reagent 2
Contains biological material
Warnings and precautions: Preservative of the reagents
Sodium azide (0.95 g/L)
Wavelength
340/410 nm
Measurement:
Kinetic
Sample/Calibrator
6.0 uL
Reagent 1
80 uL
Reagent 2
20 uL
Conversion factor
0.0167 ukat/L
Measuring range up to
600 U/L
Limit of detection
1.2 U/L
Interfering substances (with and without P-5-P)
Ascorbic acid - 30 mg/dL Bilirubin - 60 mg/dL Hemoglobin - 100 mg/dL Lipemia - 200 mg/dL
Reference range: Women
<31 U/L < 0.52 ukat/L
Reference range: Men
<35 U/L <0.58 ukat/L