Pipet Classification
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Calibration Marks/Design
• To Deliver • To Contain
Calibration Marks/Design: delivers the exact amount it holds a container
To Deliver
Calibration Marks/Design: holds the particular volume but does not dispense the exact volume
To Contain
Calibration Marks/Design: high standards for accuracy
Class A
Drainage Characteristics
• Blowout • Self-draining
Drainage Characteristics has a continuous etched rings on top of the pipet; exact volume, used for VISCOUS
Blowout
Drainage Characteristics absence of etched rings; used for NON-VISCOUS FLUID
Self-draining
Types of Pipet
a. Measuring or Graduated Pipet b. Transfer Pipet
Types of Pipet Measuring or Graduated Pipet
• Serologic • Mohr • Bacteriologic • Ball, Kolmer, Kahn • Micropipet
Types of Pipet Transfer Pipet
• Volumetric Pipet • Ostwald Folin • Pasteur Pipet • Automatic macropipets or micropipets
Measure Pipet: Graduations up to the tip Blow-out
Serologic
Measure Pipet: Graduations doesn’t reached up to the tip Self-draining
Mohr
Measure Pipet: May be Serologic/Mohr volumes ranging from 1-1000 uL To Contain
Micropipet
Transfer pipet: Bulb - middle Non-viscous Self-draining
Volumetric Pipet
Transfer pipet: Bulb - near tip w/ two etched rings Blowout
Oswald - Folin
Transfer pipet: No calibration marks ; transfer fluid without specific volume
Pasteur Pipets
Transfer pipet: Most Routinely used pipet Automated/self-operating, semi-automatic/Manually operated
Automatic Macropipets/Micropipets
Mechanisms of Automatic/Mechanical Micropipet: Relies on piston for suction Disadvantage: aerosol contamination
Air displacement pipet
Mechanisms of Automatic/Mechanical Micropipet: Operates moving piston or barrel like hypodermic syringe does not require different tip for each use Very accurate and expensive
Positive-Displacement Pipet
Mechanisms of Automatic/Mechanical Micropipet: Obtains liquid from a common reservoir
Dispenser/Dilutor Pipet
Calibration of Pipets: don’t require calibrations
Class A
Calibration of Pipets: TD pipets
Distilled water
Calibration of Pipets: TC pipets
Mercury
Calibration of Pipets: used to verify volume accuracy and precision
Gravimetric Spectrometric Photometric