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Measurement of the wavelength and the intensity of electromagnetic radiation in the visible region of the spectrum.
COLORIMETRY
Used for identification and determination of concentrations of substances that absorb light.
COLORIMETRY
relies on visual acuity to determine end-point
Visual Colorimetry
measurement of light intensity in a much narrower wavelength
SPECTROPHOTOMETRY
Makes use of prisms and/or diffraction gratings as monochromator to disperse the radiant energy into a continuous spectrum & isolate radiant energy of desired wavelength
SPECTROPHOTOMETRY
Makes use of prisms and/or ___________ as _________ to disperse the ______ into a continuous spectrum & isolate radiant energy of desired wavelength
diffraction gratings monochromator radiant energy
measurements of light intensity of multiple wavelength
FILTER PHOTOMETRY
It makes use of filters (interference or transmission) to isolate part of the spectrum
FILTER PHOTOMETRY
PRINCIPLE of SPECTROPHOTOMETRY (LAW of COLORIMETRY): ______ passes through a __________ to provide a selection of the desired region of the spectrum to be used for measurements.
Light Monochromator
PRINCIPLE of SPECTROPHOTOMETRY (LAW of COLORIMETRY): ____ are used to isolate a __________ and to improve its __________.
Slits narrow beam of light chromatic purity
The _____next passes through an __________ where a portion of the ________ is absorbed, depending upon the nature and concentration of the solution.
light absorption cell radiant energy
Any light not absorbed is transmitted to a _______, which converts the light energy to _________ that can be registered on a _____
detector electrical energy meter or a digital read-out.
The concentration of the solution is directly proportional to the amount of light absorbed and inversely proportional to the logarithm of transmitted light
BEER’S LAW
BEER’S LAW The _______ of the solution is __________ to the amount of __________ and ________proportional to the __________
concentration directly proportional light absorbed inversely logarithm of transmitted light
ratio of the radiant energy transmitted, divided by the radiant energy incident on the sample
%T
Absorbance is directly proportional to the length of light path
BOUGUER’S LAW or LAMBERT’S LAW
BOUGUER’S LAW or LAMBERT’S LAW: ___________ is _____ proportional to the _________
Absorbance directly length of light path
What is Lambert’s Law formula?
A = abc
the concentration of a solution for the known path length is directly proportional to its absorption of light.
BEER- LAMBERT LAW
BEER- LAMBERT LAW the _______ of a solution for the ________ is ____ proportional to its __________
concentration known path length directly absorption of light.
In the formula of Lambert’s law: What ‘a’ stands for?
proportionality constant or molar absorptivity or extinction coefficient
In the formula of Lambert’s law: What ‘b’ stands for?
length of light path in cm
In the formula of Lambert’s law: What ‘c’ stands for?
molar concentration of absorbing substance
provides a continuous spectrum of white light which can be separated at different wavelengths
LIGHT SOURCE