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biotechnology

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Total cards: 9•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (9)

#1
Front (Question)

what’s PCR?

Back (Answer)

Polymerase Chain Reaction • amplification of DNA • used for Gel electrophoresis

#2
Front (Question)

step 1 PCR

Back (Answer)

Denaturation Separate DNA strands by breaking hydrogen bonds (94-96C)

#3
Front (Question)

step 2 PCR

Back (Answer)

• Annealing Cool down to 50-70C

#4
Front (Question)

STEp 3 PCR

Back (Answer)

• Elongation Taq polymerase builds complementary optimum (72C)

#5
Front (Question)

how does gel electrophoresis work?

Back (Answer)

Wells always on positive side because DNA negatively charged Phosphates (sugar-ribose backbone) migrate to the positive terminal

#6
Front (Question)

what are ladders for?

Back (Answer)

isolate because it has known base pair lengths

#7
Front (Question)

whats aragose gel for?

Back (Answer)

• Permeable • Smallest fragments travel fastest • Longer fragments travel slower (negative terminal)

#8
Front (Question)

what’s sanger sequencing (- Must do PCR before sequencing)

Back (Answer)

method of sequencing: random of whether a deoxyribose or dideoribose is introduced to sequence

#9
Front (Question)

what’s ddNTPs?

Back (Answer)

randomly inserted by DNA polymerase which terminates chain elongation