Saliva Identification (Exam 3)
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This enzyme can be found in saliva at a concentration 50x higher than any other body fluid.
Amylase
In what 2 cases will you typically encounter saliva?
1) Sexual assault 2) Child abuse
What company produces the SALIgAE Test?
Abacus Diagnostics
What color change indicates a positive result for the SALIgAE Test?
Yellow
What color change indicates a negative result for the SALIgAE Test?
No color change
What 2 chemical principles should you be aware of when performing radial diffusion tests with saliva and iodine?
1) Amylase hydrolyzes starch 2) Iodine stains starch blue
What is the typical format of a saliva sample plate?
1) Undiluted saliva 2) 1:10 diluted saliva 3) 3 non-saliva fluids 4) Unknown samples i.e. evidence & stuff
What type of paper does the saliva sample plate use?
Graph paper
How much starch and agarose (percentages) should be used in the saliva sample plate petri dish?
Agarose - 1% Starch - 0.1%
What do blue areas on the saliva plate indicate?
Starch present; amylase absent
What do clear areas on the saliva plate indicate?
Amylase present; starch absent
Assume that the diameter of the evidence (clear circle) is greater than the reference (clear circle) in a saliva plate. What does this mean?
There is an elevated level of amylase; therefore, saliva is present