ENHANCEMENT CLASS: FUNDAMENTAL CONCEPTS IN ANALYTICAL PROCEDURES

• Facilitate the correct performance of analytic procedures that yield accurate and precise information, aiding patient diagnosis and treatment.

Use to measure the following scales

Basic unit of temp in SI system

3 Major types of Thermometers:

• Uses colored liquid encased in plastic material with a bulb at one end • Measures temp between 20 and 400° C

Accurate and fast-reading Advantages: SIZE and MILLISECOND RESPONSE TIME

Vessels holding/transferring liquid are designed either to contain or deliver a specified volume.

Borosilicate

Aluminosilicate

Acid and alkali resistant

Plasticware

Designed to hold different volumes rather than one exact amount.

Are glass utensils used to transfer liquids.

DESIGN

Do not deliver the exact/same amount

• Referred as “rinse-out pipettes” • Calibration medium - mercury • Ex. WBC Pipet , SAHLI Pipet, RBC Pipet

Delivers the exact/same amount

• Designed to drain by gravity • Calibration medium - Distilled water • Ex. Serological Pipet, volumetric, Mohr pipet (the tip should remain in contact with the side of the vessel for several seconds after the liquid has drained.)

Drainage Characteristics

• Has “etched rings” located near the top of the pipet • The last drop should be blown out to obtain the exact volume of liquid.

• No etched rings • Designed to drain liquid by gravity • The tip of the pipet should not be in contact with accumulating fluid in the receiving vessel during drainage.

TYPES

Measuring or Graduated pipettes

Transfer pipettes