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Lecture 21 - Methods of protein analysis II

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卡片总数: 7内容版本: v3公开卡包更新时间: 8/1/2026

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#1
正面 (问题)

Acid hydrolysis is a method to determine

背面 (解答)

the amino acid composition of a protein

#2
正面 (问题)

Edman degradation is used to

背面 (解答)

determine the amino acid sequence

#3
正面 (问题)

Edman degradation sequentially

背面 (解答)

removes one residue at a time from N-terminal of peptide

#4
正面 (问题)

Enzymatic fragmentation is used because

背面 (解答)

it can be difficult to measure large proteins

#5
正面 (问题)

Trypsin cleaves

背面 (解答)

on the C-terminal side of lysine and arginine

#6
正面 (问题)

Cyanogen bromide acts only on

背面 (解答)

methionine residues

#7
正面 (问题)

Mass spectrometry of peptide fragments is an alternative to

背面 (解答)

Edman degradation