LIVER FUNCTION TEST part 3

What is the average daily bilirubin production?

85% of bilirubin is derived from this.

15% of __________________ destroyed in the BM

What are the protein involved in the catabolism of other heme?

What ate the forms of Bilirubin?

bound by albumin & transported to the liver; water - insoluble

orange-yellow pigment derived from the hemoglobin degradation, it is extracted and bio transforming in the liver and excreted in the bile and urine

It is the main transporter of Unconjugated Bilirubin

It is a subtype of CB wherein it is none-polar, water insoluble and referred to as “indirect bilirubin” or hemobilirubin.

What is the other name of B1?

formed from the reaction of UCB with glucuronic acid; polar and water soluble

Conjugated Bilirubin is AKA as?

It is AKA ‘direct bilirubin”?

conjugated bilirubin bound to Albumin

Introduced the Diazo reaction for the detection of Bilirubin using urine

In the Diazo reaction, what acid is reacted with bilirubin?

applied the Diazo reaction in serum in the presence of an accelerator (solubilizer)

discovered the polar (direct reading) &non-polar (indirect reading) types of bilirubin in serum

50% CH3OH as the non-polar solvent; first useful quantitative method for bilirubin

used the Diazo reaction with caffeine-benzoate-acetate as an accelerator

Published a candidate reference method for Total Bilirubin: _____________________?

Agencies for the methods used?

 both have acceptable precision  adapted by many automated instruments  most frequently used methods to measure bilrubin

What is the color of the diazo product in acid pH?