Amino acids, Proteins and DNA
No description yet. This curated deck is ready for daily study.
Card preview (24)
What 2 groups does an amino acid contain
Carboxyl group Amino group
Which part of an amino acid reacts with a base
Carboxyl group
Which part of an amino acid reacts with an acid
Amino group
Write an equation for the reaction between 2-aminopropanoic acid and H+
Write an equation for the reaction between 2-aminobutanoic acid and NaOH, name the organic product formed
What is the name of the point at which amino acids are neutral
Isoelectric point
What determines how acidic or basic an amino acid is
The R group attached, Alkyl groups are electron releasing Acyl groups are electron withdrawing
How do amino acids exist in neutral solution
As zwitterions
How do amino acids exist in a solution of low pH
NH2 gains H+ to become NH3+
How do amino acids exist in a solution of high pH
COOH looses H+ to become COO-
Describe the structure and melting point of amino acids in the solid form
They have crystalline structures with high melting points due to the strong electrostatic forces of attraction than must be overcome between the zwitterions
What occurs when an amino acid reacts with an alcohol in the presence of conc H2SO4
• The carboxyl group undergoes esterification • NH2 gains a proton from the sulfuric acid
Write an equation for the reaction between 2-aminopropanoic acid and methanol in the presence of H2SO4
Which part of an amino acid reacts with an acyl chloride
Amino group
Which part of an amino acid reacts with an acid anhydride
Amino group
What is the major product when we react an amino acid with excess halogenoalkane
Quaternary ammonium salt
Outline the stages in TLC chromatography for amino acids
Wear plastic gloves to hold onto the TLC plate to prevent contamination of hands to plate Draw pencil line 1.5cm from bottom of plate Use capillary tube to apply small drop of amino acid to mid point of pencil line Allow spot to dry Add solvent of depth 1cm (must be below pencil line or will dissolve mixture from plate) Place TLC plate in developing tank Allow developing solvent to rise up the plate (not reaching the very top) Remove plate and quickly mark position of solvent front with pencil mallow to dry in fume cupboard (solvent may be toxic) Spray with developing agent Ninhydrin Measure distance from initial pencil line to spots (A) Measure distance from initial pencil line to solvent front (B) Calculate RF values (A/B)