mboc - week 2

No description yet. This curated deck is ready for daily study.

Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (24)

Front (Question)

Epigenetic regulation

Back (Answer)

Heritable change in gene expression or genome function, door mitose gene expression and expression states (vooral planten) veranderlijk. Door meerdere delingen doorgeefbaar maar omkeerbaar

Front (Question)

If epigenetic regulation goes wrong consequences

Back (Answer)

disturbed development, diseases, transgene silencing, faster aging

Front (Question)

In ratten epigenetische regulatie invloed –

Back (Answer)

likken geeft verwijdering methylatie en meer expressie glucocorticoid receptor (stress)

Front (Question)

Epigenetische modificaties

Back (Answer)

• Noncoding RNA • Histone variant (structure nucleosome) • Histone modifications • DNA methylations

Front (Question)

Bekende mutaties bij chromatinesoorten:

Back (Answer)

Heterochromatin: H3K9me Euchromatin active: H3K4me3 Euchromatin inactive: H3K27me

Front (Question)

Genome always off modifications;

Back (Answer)

DNA methylation, H3K9methylation, transposons

Front (Question)

Genome active temporarily modifications;

Back (Answer)

RNAP2, activator, H3k4methylation, histonacetylation, genes

Front (Question)

Genome inactive temporarily modifications;

Back (Answer)

H3K27, repressor proteins, genes

Front (Question)

euchromatine wanneer open en wanneer niet

Back (Answer)

H3ac, H3K4me, activator/trithorax protein open repressor/polycombgroup (PcG) proteins, H3K27me dicht

#10

Front (Question)

wat nodig naast epigenetische modificatie -

Back (Answer)

sequence specific transcription factors en chromatin remodelling nodig omdat niet alles in een keer open moet

#11

Front (Question)

Transposable element

Back (Answer)

change position in genome, regulates gene expression, can multiply itself

#12

Front (Question)

Saccharomyces cerevisiae voordelen en nadelen

Back (Answer)

deelt snel en aseksueel, makkelijk onderhouden, homologous recombinations, tiny genome geen DNA methylation, H3K9methylation, geenH3K27 methylation, no RNAi, unicellar, few TE and distal CREs

#13

Front (Question)

Schizosaccharomyces pombe voordelen en nadelen

Back (Answer)

H3K9me, RNAi geen DNA methylation, H3K9methylation, geen H3K27 methylation, unicellar, few TE and distal CREs

#14

Front (Question)

Caenorhabditis elegans voordelen en nadelen

Back (Answer)

H3K27me/polycomb silencing, multicellular geen DNA methylation, few TE and distal CREs

#15

Front (Question)

Drosophila melanogaster voordelen en nadelen

Back (Answer)

betekenisvolle phenotypische veranderingen geen DNA methylation, few TE and distal CREs

#16

Front (Question)

Arabidopsis thaliana voordelen en nadelen

Back (Answer)

DNA methylation, lots of variants not as many TEs as organisms with large genome

#17

Front (Question)

Wat is DNA methylation, waar geplaatst

Back (Answer)

5-methylcytosine (5mc) in meeste eukaryoten, vooral cytosine in CG context. * Transposable elements have DNA methylation, samen met H3K9 * CpG rich ‘islands’/Promoters are unmethylated * Protein-coding regions have DNA methylation in gene body Heel groot deel 5mc in transposons en repetitief DNA trekt DNAmethyl binding proteins (MBPs) aan

#18

Front (Question)

5hydroxymethylcytosine

Back (Answer)

zorgt voor activaite enhancers, eerste stap in verwijderen 5mc voorkomt MeCP2 binding en TET voorkomt DNMTs

#19

Front (Question)

Methylated cytosines ways of repression

Back (Answer)

Inibition of TF die RNAP stimuleren Methyl binding proteins (MBPs) bind 5mC & recruit repressor proteins (e.g. H3-K9 methyltransferase) to repress transcription DNA methyltransferases (DNMTs) are bound to histone deacetylases (HDACs) and histone methyltransferases (HMTs), resulting in transcription inactivation

#20

Front (Question)

Methylation meten voor specifieke regios waarmee

Back (Answer)

• DNA blot analyse • qCPR • bisulfite sequencing

#21

Front (Question)

genome wide methylatie meten waarmee

Back (Answer)

• immunolabeling • IP met 5mc antilichaam samen met qPCR/microarray/sequencing (me-DIP) • Sequencing DNA methylation libraries • Genome wide bisulfite • Genome wide enzymatic methyl seq

#22

Front (Question)

MeDIP

Back (Answer)

gefragmenteert DNA, 5mc antilichaam er bij en groen fluor, ander deel rood (dit is niet gemethyleerd), magnetische beads om die er weer uit te halen, daarna sequencen om te kijken welke sequences zijn enriched

#23

Front (Question)

Bisulfite sequencing

Back (Answer)

analysis of methylation of every cytosine in DNA. Bisulfite changes unmethylated cytosines into uracil. Gemethyleerd blijft C, niet omschrijven naar dochterstrand!! ACTA BLIJVT AUTA!! PCR en cloning voor klein fragment of sequencing voor heel genoom. Als je top en bottom strand vergelijkt bij dochtercel zie je dat code verandert.

#24

Front (Question)

Methylation 5mc afwijkende targets

Back (Answer)

• Normaal 5’–CG–3’ • In embyryo; 25% non CG, mainly CA • Mammalian brain 1.5% non CG, mainly CA • Planten; CG, CHG (H = A,T or C) which is symmetric or CHH which is asymmetric because one C methylation is missing