SPECIMEN COLLECTION & HANDLING
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Conscious Inpatients/ Hospitalized patients
Verbally ask their full names including middle names Verify using ID bracelet which includes: 1. First & Last names 2. Hospital/Unit # 3. Room/ Bed # 4. Physician’s name
Sleeping patients
Identified in the same manner as conscious in-patients Must be awakened before blood collection
Unconscious, Mentally Incompetent Patients
Identified by asking the attending nurse or relative As well as thru ID bracelet
Infants & Children
A nurse or relative may identify the patient
Outpatient/ Ambulatory Patient
Verbally ask: 1. Full names 2. Address 3. Birth date Countercheck with Valid ID with photo
3-Way ID to avoid misidentification:
• Patient’s verbal ID statement • Check ID band • Visual comparison of labeled specimen with patient’s ID before leaving bedside
Average human body contains how many liters of WB
5 quartz or 4.73 L
Adult Males have approximately how many liters of WB
5 - 6 L
Adult Females have approximately how many liters of WB
4 - 5 L
WB is composed of ___ Plasma and ___Cells
60%; 40%
Process by which blood is obtained from a pateint’s artery
Arterial Puncture UseL Blood gas analysis & pH measurement
Sites for Arterial Puncture (5)
• Radial Artery • Brachial Artery • Femoral Artery • Scalp Artery • Umbilical Artery
This test is performed prior to blood collection from radial artery
Modified Allen Test
Major Complication of Arterial Ouncture:
Thrombosis Hemorrhage Possible Infection
Unacceptable Sites:
Irritated Edematous Near a Wound Area of an AV shunt or Fistula
Angle for Arterial Puncture:
45 - 60 degrees
Blood is obtained from a patient’s vein Deoxygenated blood with a dark red color
Venipuncture
Sites for Venipuncture:
Antecubital fossa region Veins on the wrist & dorsal aspect of hands Veins on ankle
Antecubital fossa region sites:
• Median Cubital vein (best site) • Cephalic Vein • Basilic Vein (not chosen unless no other vein is more prominent; near brachial artery)
Order of Draw
• Yellow • Light Blue • Serum (Red) • Green • Lavender/ Purple • Gray
Anticoagulant based on Order of draw
• Blood Culture Tubes, Sodium Polyanetholesulfonate (SPS) • Trisodium Citrate • With or Without Clot Activator or Gel Separator • Heparin (Li, Na, NH4) • EDTA • Sodium fluoride & Potassium Oxalate
What tube is used for Lactate Sample collection?
Gray tube Blocks Glycolysis
Tourniquet placement and duration
3-4 inches above the site Never leave tourniquet longer than one minute
Angle of needle on Venipuncture
15 to 30 degree angle Bevel side up*