Module 6:analysis
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Chromatography What is chromatography
Analytical technique used to separate and identify component moleucles of a mixture
Chromatography What are the two phases
Mobile Stationary
Chromatography What is the mobile phase
Fluid that allows molecules to move over or through the stationary phase such as liquid or gas
Chromatography What’s true for species thaty are more soluble in the mobile phase
Move further or faster within mobile phase
Chromatography What is the stattionary phase
Substance that has afffinity to it oleucles in the mixture being analysed
Chromatography What dies a greater affinity to the stattionary phase mean
Shorter the distance or slower moves within the mobile phase
Chromatography What is rf value
Retention factor that is unique to each different component moleucles in the mixture being analysed
Chromatography How do you calculate rf
Distance moved by molecule / distance moved by solvent Al;ways less than 1
Chromatography Why does rf change
Different moleucles have different affinities for mobile stattionary phase
Chromatography What are types of chromatography
TLC Liquid Gas Hplc
Chromatography What happens in tlc
Metal plate coated in thin layer of silica sample dotted on plate paced above level of solvent Solvent moves up plate separating substances Dried then chemical traces viewed using uv lamp or using developing agent
Chromatography What happens in gas chromatography
Thin coiled tube packed with a solid powdered substance that acts as the stationary phase high pressure gas passed through tube and acts as mobile phase Machine records a retention time for each component in the mixture