METHODS FOR GLUCOSE MEASUREMENT & OTHER CLINICAL CORRELATIONS

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Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (24)

Front (Question)

Chemical methods for measuring glucose levels:

Back (Answer)

• Oxidation-reduction methods • Condensation method (Dubowski)

Front (Question)

Alkaline copper reduction methods:

Back (Answer)

• Folin-Wu • Nelson-Somogyi • Neocuproine method • Benedict’s method

Front (Question)

Cu + phosphomolybdate —-> PM blue (phosphomolybdenum) what alkaline copper reduction reaction is shown above?

Back (Answer)

Folin-Wu method

Front (Question)

Cu + arsenomolybdate —-> AM blue (arsenomolybdenum) what alkaline copper reduction reaction is shown above?

Back (Answer)

Nelson-Somogyi method

Front (Question)

Enzymes used in measuring glucose levels:

Back (Answer)

• Glucose oxidase • Hexokinase

Front (Question)

Most specific enzyme reacting with only B-D-glucose:

Back (Answer)

Glucose oxidase

Front (Question)

Two ways in which glucose oxidase can be measured:

Back (Answer)

• Colorimetric method • Polarographic method

Front (Question)

Measures the rate of disappearance of oxygen using oxygen electrode in glucose oxidase method:

Back (Answer)

Polarographic method

Front (Question)

Glucose oxidase method that uses a side reaction that uses H2O2:

Back (Answer)

Colorimetric method

#10

Front (Question)

What enzyme is used in the side reaction for colorimetric method?

Back (Answer)

Peroxidase

#11

Front (Question)

This method is more accurate than glucose oxidase methods, because the coupling reaction using G6PDH is highly specific:

Back (Answer)

Hexokinase method

#12

Front (Question)

What is measured in Hexokinase method?

Back (Answer)

NADPH

#13

Front (Question)

NADPH is has a strong affinity and measured at the wavelength of:

Back (Answer)

340 nm (UV)

#14

Front (Question)

Is Hexokinase method affected by ascorbic acid or uric acid?

Back (Answer)

NO. The hexokinase method is NOT affected by ascorbic or uric acid.

#15

Front (Question)

Most common cause of lactose intolerance:

Back (Answer)

Lactase deficiency

#16

Front (Question)

Why does lactase intolerance causes watery diarrhea?

Back (Answer)

Lactose is not digested. Lactose retains water

#17

Front (Question)

Glycogen storage disease with a defective enzyme glucose-6-phosphatase:

Back (Answer)

Von Gierke Disease

#18

Front (Question)

Enumerate Glycogen Storage Diseases:

Back (Answer)

• Type I - Von Gierke • Type II - Pompe • Type III - Cori • Type IV - Andersen • Type V - McArdle • Type VI - Hers • Type VII - Tarui disease Mnemonic: “Viagra Pill Causes A Muscle Hardening Tight”

#19

Front (Question)

What type of glycogen storage disorder wherein the defective enzyme is a-1,4-glucosidase:

Back (Answer)

Type II Pompe

#20

Front (Question)

Defective enzyme in type III (Cori) glycogen storage disease:

Back (Answer)

debranching enzyme

#21

Front (Question)

Defective enzyme in type IV (Andersen) glycogen storage disease:

Back (Answer)

Branching enzyme

#22

Front (Question)

Organ affected in Type V (McArdle) glycogen storage disease:

Back (Answer)

Muscle

#23

Front (Question)

Organ affected in Type VI (Hers) glycogen storage disease:

Back (Answer)

Liver

#24

Front (Question)

Enzyme defective in Type VII (Tarui) glycogen storage disease:

Back (Answer)

Phosphofructokinase