Lipase (M)
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What is the meaning of LPS?
Lipase
What is LPS?
It is an enzyme that hydrolyzes fats
What is the difference between LPS and AMS?
LPS is more sp > AMS
What is the fxn of LPS?
It is used to diagnose acute pancreatitis
• If the pt has acute pancreatitis, what happens to the pt’s LPS lvls in the ff conditions? 1) When does it rise? 2) When does it remain elevated?
1) LPS lvls rise 4 - 8 hrs 2) LPS lvls remains elevated for 8 - 14 days
What are the tissue sources of LPS?
1) Pancreas 2) Stomach 3) Small intestine
What is the principle employed for determination of LPS lvls?
Turbidimetric
What is the principle of rxn for determination of LPS lvls?
Triglyceride + H2O -> mono. + di-glycerides + fatty acids substrate: 0.8% (w/v) olive oil in alcohol
What is the process (or steps) for determination of LPS lvls?
1) Label 3 test tubes: BLANK, STANDARD, UNKNOWN 2) Place 3,000 uL of LPS working rgnt 3) Pipette 100 uL sx 4) Incubate for 5 mins 5) Read and record A at 400 nm
What is the normal reference range of LPS lvls for adults?
10 - 150 U/L
What is the normal reference range of LPS lvls for old individuals (60 yo >)?
18 - 180 U/L