Lecture 9
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DNA –> RNA
Transcription bacteria and archae
RNA-> Protein
Translation bacteria and archae
RNA vs. DNA
Ribose/Deoxyribose Sinlge/Double Uracil/Thymine
mRNA
Translated into protein. Little 2 structure unstable. t1/2~ 5 min E. coli.
rRNA
Not translated into protein. “Non-translated genes.”
tRNA
Not translated into protein. “Non-translated genes.”
T or F RNA polymerase doesnt need a primer?
T. unlike DNA polymerase in replication RNA polymerase does not need a primer to start sythesiing RNA 5’-3’.
RNA polymerase
Five subunits 2 alpha, Beta, Beta’, Sigma, and Omega.
Omega subunit
Required for assembly of the core enzyme
Eukaryotes have how many RNA polymerase?
• Which transcribe different types of RNAs
Bacteria have how many RNA polymerase?
1.activity is modualted by sigma factors.
Promoters extend __bp upstream of the transcription site
250 bp
Promoters are regions that allow ____ enzyme to bind
RNA polymerase
Promoters contain 2 sequence motifs at
-10 and -35
Promoters also bind DNA-binding proteins which control ____
Gene expression
Promoters are ____ of the gene they control and the gene is ____ of its promoter
upstream/downstream
What specifies the site of transcription initiation by recognizing a promoter?
Sigma Factor
Purine is usually a ____ site
Transcriptional start
Sigma 70
Houskeeping genes
Sigma 54
Nitrogen starvation genes
Sigma 38
Stationary phase genes
elongation
sigma leaves and transcription continues until transcriptional terminator is reached.
In prokaryotes several genes may be ____ to form ____ mRNA. Those genes are organized into an ____
Co-transcrobed/ polycistronic/operon
Termination of transcription can occur after the mRNA and forms a ____ followed by a poly __ tail.
stem loop/ A or U