Unit 2 Review
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Electromagnetic radiation exists both as Maxwell’s wave and as streams of particles called:
Photons
Amplitude of the electro magnetic sine wave is defined as:
• the length of the electronic vector at maximum peak height
A nano-meter is equivalent to:
10-9 meters
What is the concentration of serum uric acid in a solution whose absorbance is 0.098, cuvette path length, 1.0 cm, absorptivity is 0.102 L g-1cm-1?
0.96 mg/L
What is the concentration of creatinine in a serum sample if given the following? A test = 0.150 A standard = 0.90 C standard = 1.0 mg/dL
0.167 mg/dL
Which of the following type of light sources emits radiation that changes in intensity very slowly as a function of wavelength?
• continuum source
Filters and diffraction gratings are examples of which of the following?
Monochromators
The wavelength in nanometers at peak transmittance is termed:
Nominal wavelength
Bandpass is defined as:
The total range of wavelengths transmitted
A photomultiplier tube functions to:
Increase the electronic signal produced as photons cascade from dynode to dynode
Didymium and holmium oxide filters are used to assess:
Wavelength
Which of the following is a cause of stray light failure in a spectrophotmeter?
Room light entering the spectrophotometer and impinging upon the detector
Light reflecting off a urine dipstick absorbent surface is an example of which the following?
• diffuse reflectance
What of the following describe atoms absorption spectroscopy?
The absorption of monochromatic electromagnetic radiation by an element in its ground state with a net zero charge
Which of the following statements best illustrates the difference between photons from fluorescent emission and the excitation photons?
Emission photons are lower energy and longer wavelengths
The polarization of light in fluorescent polarization immunoassay allows the measurement of which of the following?
Bound fraction in the presence of the free fraction
A chelate of europium (Eu3+) is used in which of the following assays?
Time-resolved fluorescent immunoassays
Oxidation of acridinium esters by hydrogen peroxide in the presence of peroxidase produces which of the following?
Chemiluminescence
Turbidimetry is the measurement of the reduction in light transmission caused by:
Particle formation
The presence of particulates in a urine same measured by a refractometry will result in which of the following?
A change in the critical angle of the light as it passes through the solution
Which of the following accounts for the temperature rise in freezing point osmometry?
Heat of fusion
Which of the following electrical devices senses the freezing point of a solution in an osmometer?
A thermistor
Through is defined as:
Number of tests performed per hour
Which of the following statements best describes discrete testing?
Measure only the test requested on a sample