Experiment#5: Chromatography
No description yet. This curated deck is ready for daily study.
Card preview (24)
Used to separate mixtures of compounds and identify an unknown.
Chromatography
Exploits the differences in solubility and adsorption
Chromatography
CHROMATOGRAPHY A name/name of the phase for the porous material used in chromatography.
Adsorbent or Stationary Phase
CHROMATOGRAPHY A name/name of the phase for the solvent that moves the mixture through the adsorbent.
Eluent or Mobile Phase
CHROMATOGRAPHY Uses a solid stationary phase and a liquid mobile phase, wherein separation is governed by the surface adsorption phenomena.
Adsorption Chromatography
Refers to the ability of a substance to “stick” to a surface.
Adsorption
ADSORPTION CHROMATOGRAPHY Molecules of interest which are getting adsorbed on the adsorbent.
Adsorbate
ADSORPTION CHROMATOGRAPHY The extent of adsorption of a single component depends on which 3 factors?
• Polarity of the Molecule • Activity of the Adsorbent • Polarity of the Liquid Mobile Phase
CHROMATOGRAPHY Uses a liquid stationary phase supported on the surface of a solid and a liquid or gas mobile phase which is insoluble in the stationary phase.
Partition Chromatography
CHROMATOGRAPHY This type of chromatography is governed by differences in the solubility of the sample in the stationary and mobile phase.
Partition Chromatography
PARTITION CHROMATOGRAPHY Separation is based on _______ ________ between two liquid phases.
Solute Partitioning
CHROMATOGRAPHY A type of solid-liquid adsorption chromatography that uses a thin layer of adsorbent supported on a flat surface as the solid phase.
Thin Layer Chromatography
THIN LAYER CHROMATOGRAPHY What are the 2 possible adsorbents found on the flat surface of a piece of glass or paper used as the solid/stationary phase?
• Alumina • Silica Gel
THIN LAYER CHROMATOGRAPHY A polar adsorbent that can hydrogen bond.
Silica Gel
TRUE or FALSE | THIN LAYER CHROMATOGRAPHY Polar components of the mixture will interact less with the silica gel and move faster up the TLC Plate.
FALSE. Polar components will interact more, and move less quickly.
TRUE or FALSE | THIN LAYER CHROMATOGRAPHY Non-polar compounds will move faster and migrate further up the TLC Plate.
TRUE
THIN LAYER CHROMATOGRAPHY Provide at least 3 (or all) of the 5 purposes of Thin Layer Chromatography.
• Identify compounds/unknown mixtures • Detect intermediates in reactions • Determine the progress of a reaction • Determine the number of components in a mixture • Evaluate the efficiency of purification processes
CHROMATOGRAPHY Technique for separating dissolved chemical substances by taking advantage of their different rates of migration across sheets of paper.
Paper Chromatography
CHROMATOGRAPHY Used to separate and identify amino acids in a mixture.
Paper Chromatography
PAPER CHROMATOGRAPHY Made of highly purified cellulose that absorbs and retains water (hydrophilic), serving as the stationary phase.
Filter Paper
PAPER CHROMATOGRAPHY What is the solvent system for Paper Chromatography? (Hint: 1:2 (v/v) 2% ______ _______-______ _______)
1:2 (v/v) 2% ammonium hydroxide-isopropyl alcohol
PAPER CHROMATOGRAPHY The hydrophic organic solvent is drawn up the paper by ….?
Capillary Action
PAPER CHROMATOGRAPHY A point where the sample is initially spotted on the paper.
Baseline or Origin
PAPER CHROMATOGRAPHY The distance moved by the solvent along the paper.
Solvent Front