Chapter 10
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probe
segment of single-stranded nucleic acid used to identify DNA molecules containing complementary sequence
template strand
DNA strand that is transcribed to create RNA transcript OR strand of RNA used to create complementary RNA
consensus sequences
short stretches of DNA that appear in many different genes w/little variation
termination
end of transcription or translation
initiation complex
combination of small ribosomal subunit, mRNA, molecule, and tRNA charged w/first amino acid coded for by the mRNA; formed at start of translation
small nuclear ribonucleoprotein particle (snRNP)
enzyme + small nuclear RNA molecule, functioning in RNA splicing
poly A tail
50-250 adenine nucleotides added after transcription to 3’ end of eukaryotic mRNAs
RNA splicing
last stage of RNA processing in eukaryotes – intron transcripts excised via snRNP
polyribosome
complex: mRNA + ribosomes; ribosomes synthesize polypeptide chains as proceed
transcription
synthesis of RNA using one strand of DNA as template
coding strand
one of two strands of DNA that for a particular gene specifies amino acids
translation
protein synthesis on ribosomes using info encoded in messenger RNA
peptidyl transferase
catalytic function of large ribosomal subunit: breaking bond b/w tRNA in P site, forming polypeptide bond b/w amino acid and amino acid attached to tRNA in A site
nucleic acid hybridization
single-strand nucleic acid probe made that binds to target DNA or RNA, resulting in hybrid
mRNA
messenger RNA - transcript of DNA region, carries information as codons
transcription initation site
part of gene’s promoter where synthesis of gene’s RNA transcript starts
ribosome
site of protein synthesis
spliceosome
RNA-protein complex that splices out introns from pre-mRNA
start codon
AUG - mRNA triplet acting as signal for translation
ribosomal RNA (rRNA)
type of RNA involved in peptide bond formation; incorporated into ribosome
G cap
chemically modified GTP added to 5’ end of mRNA - facilitates binding of mRNA to ribosome
stop codon
UAG, UGA, UAA - signal end of translation
transfer RNA (tRNA)
carries amino acid and anticodon that pairs w/codon in mRNA during ranslation
intron
portion of gene w/in coding region transcribed into pre-mRNA but spliced out