Proteins part 5
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• Causes y-globulins to migrate toward the cathode even though they are slightly (-) charged • Due to electrical charge on support medium
ELECTROENDOSMOSIS
• Relative concentration of each bond determined by _____________?
densitometry
Plasma samples result in fibrinogen peak between gamma and beta fractions
Specimen collection and handling
• Due to IgA (in serum); Cirrhosis • Seen in patients with liver cirrhosis cause by increase in IgA
Beta-gamma bridging effect:
• Monoclonal gammopathy (Multiple myeloma)
Monoclonal band (gamma-globulin);
• Chronic inflammation
Poluclonal band
• nephrotic syndrome
Increase in a-2-macroglobulin
deficiency in a-a-antitrypsin; Juvenile cirrhosis
a-1-globulin flat curve:
• Seen in patients with Monoclonal gammopathy
Gamma spike:
• Seen in patients with emphysema
• A-1-antitrypsin
• Increased beta region, use plasma instead of serum.
• A-1-antitrypsin
What is the reference range for total protein and Albumin?
• Total protein: 6.5 – 8.3g/dL • Albumin: 3.5 – 5.5g/dL