PHOSPHATASES
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from what class is alkaline phosphatase
hydrolases
systematic name of alp
Orthophosphoric Monoester Phosphohydrolase (Alkaline Optimum)
product of alp
alcohol phosphate ion
optimum pH of alp
9.0 -10.0
activators of alp
magnesium zinc
substrate of bessey-lowry-brock method
p-nitrophenylphosphate
p-nitrophenyl phosphate (colorless) is HYDROLYZED to
p-nitrophenol (yellow)
Calculation of ALP activity based on the molar absorptivity of p-nitrophenol
BOWERS AND MCCOMB
CONTINUOUS-MONITORING TECHNIQUE is …proportional to alp activity
DIRECTLY PROPORTIONAL
tissue sources of alp
liver bone kidney spleen intestine placenta
absorbance of alp
405 nm
major tissue source of acp
prostate
most anodal furthest to cathode
liver alp
most heat labile
bone alp
least heat labile
placenta alp
least anodal nearest to cathode
intestinal alp
isoenzymes of acp
prostatic lysosomal macrophage erythrocytic osteoclastic
elevated during adenocarcinoma of the pancreas
nagao alp
most heat stable alp (in general)
regan alp
abundant in cancers affecting the lungs, breasts, and gynecological areas
regan alp
important in differentiating bone alp and regan alp
phenylalanine reagent
major source of acp
prostate
what sample that men produce where acp is found
semen
most common method of measuring alp kinetic method
bowers and mccomb