Quiz 3

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Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

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Front (Question)

Specimen Preparation

Back (Answer)

Fresh and adequately preserved Well mixed

Front (Question)

What is the purpose of a microscopic examination?

Back (Answer)

To detect, identify, and semi-quantitate formed elements in the urine sediment

Front (Question)

Specimen Volume

Back (Answer)

10-15 ml in conical tube (usually 12 ml)

Front (Question)

Centrifugation

Back (Answer)

Do not use brake and cap all specimens usually 1500 rpm for 5 minutes

Front (Question)

Sediment Preparation

Back (Answer)

Leave volume of 1.0 ml or 12:1 concentration Aspirate supernate with pipette Gently resuspend the sediment

Front (Question)

Volume of Sediment Examined

Back (Answer)

Traditional – 1 drop with 22x22 glass cover slip; avoid overflow outside cover slip

Front (Question)

Examination of Sediment

Back (Answer)

Bright field most common microscopy used Reduce light and use continuous fine adjustment focusing Exam under power (100x) at least 10 field Identify using high power (400x) for at least 10-15 fields

Front (Question)

Kova Stain • Chemicals • Identification

Back (Answer)

Most frequently used • Crystal violet and Safranin O • Identify WBCs, Epithelial, and Casts

Front (Question)

2% Acetic Acid • Purpose • Identification

Back (Answer)

• Lyses RBCs 2. Distinguish WBC, yeast, oil droplets, and crystals

#10

Front (Question)

Lipid Stains • Chemicals • Identifies

Back (Answer)

• Oil Red O and Sudan III 2. Stains Triglycerides and neutral fats orange-red, but does not stain cholesterol

#11

Front (Question)

Prussian Blue Stain 1. Stain

Back (Answer)

• Stain hemosiderin blue

#12

Front (Question)

Cytodiagnostic Urine Testing • What department? • Type of stain? • Clinical Significance

Back (Answer)

• Cytology Department • Papanicolaou Stain • Renal disease and malignancies of lower urinary tract

#13

Front (Question)

What is the normal value for RBC in the urine sediment?

Back (Answer)

0-2 RBC / hpf

#14

Front (Question)

What is the normal value for WBC in a urine sediment?

Back (Answer)

0-4 / hpf

#15

Front (Question)

What is the normal value of epithelial cells in the urine sediment?

Back (Answer)

Few epithelial cells

#16

Front (Question)

What is the normal value of hyaline casts in a urine sediment?

Back (Answer)

0-2 Hyaline casts / lpf

#17

Front (Question)

What is the normal amount of mucus found in a urine sediment?

Back (Answer)

Trace amounts

#18

Front (Question)

How do you calculate the amount of red blood cells in a microscopic?

Back (Answer)

Calculate average # in 10 hpf

#19

Front (Question)

Crenated Red Blood Cells

Back (Answer)

Hypersthenuric –> high osmotic pressure in urine

#20

Front (Question)

Ghost Cells

Back (Answer)

Lysed RBCs with only cell membrane left –> result of acetic acid

#21

Front (Question)

Dysmorphic Red Blood Cells

Back (Answer)

Indicative of glomerular damage Vary in size and cellular background

#22

Front (Question)

Hematuria

Back (Answer)

Presence of RBC in urine

#23

Front (Question)

What does hematuria indicate?

Back (Answer)

Renal disease; bleeding in urinary tract; infection; tumor; strenuous exercise; trauma; inflammation; stones; catheterization

#24

Front (Question)

Pyuria

Back (Answer)

Presence of WBC in urine