Carbohydrates 2 pt.2
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Measurement of Glycosylated Hemoglobin (HbA1C) _ has no effect on the test
Dietary status
Measurement of Glycosylated Hemoglobin (HbA1C) Specimen:
EDTA Whole blood
Measurement of Glycosylated Hemoglobin (HbA1C) Methods:
Electrophoresis, Immunoassay, HPLC, and Affinity Chromatography
Measurement of Glycosylated Hemoglobin (HbA1C) Interferences:
Alcohol, Vitamins (Retinol and Ascorbate), Drugs (Salicylates and Opiates), and abnormal hemoglobins (HbF, HbS, HbC)
Measurement of Glycosylated Hemoglobin (HbA1C) Interpretation of Results: ○ _ indicates a normal HbA1C result
<5.7%
Measurement of Glycosylated Hemoglobin (HbA1C) ● Interpretation of Results: indicates increased risk of diabetes (pre-diabetes)
5.7 - 6.4%
Measurement of Glycosylated Hemoglobin (HbA1C) ● Interpretation of Results: ○_ on at least two occasions is indicative of DM
> 6.5 % (more than or equal)
Reflects short-term glucose control over 2–3 weeks
Fructosamine
Useful for diabetics with hemolytic anemia, Hb variants (S or C), or shortened RBC lifespan.
Fructosamine
Not recommended if plasma albumin <30 g/L, as it may underestimate levels
Fructosamine
Fructosamine Methods:
Chromatography, enzymatic, and immunoassay
Fructosamine Reference range:
205 - 285 umol/L
It is a congenital deficiency of one of the three enzymes involved in galactose metabolism
Galactosemia
Galactosemia Three enzymes:
○ Galactose 1-phosphate uridyl transferase ○ Galactokinase ○ Uridine diphosphate galactose-4-epimerase
Clinical feature: Jaundice, hepatomegaly, easy bruisability, galactosuria, E. coli sepsis, cataract, hypotonia, and sensory neural defects
Galactosemia
Galactosemia Diagnostic test:
Erythrocyte galactose-1-PO4 uridyl transferase activity
Intravenous Galactose Tolerance Tests ● Done for _ (decreased glucose levels)
Type 1 Glycogen Storage Disease
Intravenous Galactose Tolerance Tests Serial blood specimens are collected for _ _ interval for testing
2 hours at 15 minutes
Essential Fructosuria ● It is an autosomal recessive disorder characterized by
fructokinase deficiency
Fructokinase catalyzes the conversion of fructose to fructose-1- phosphate
Essential Fructosuria
Essential Fructosuria Diagnostic test:
presence of fructose in the urine
A defect in fructose-1,6-bisphosphate aldolase activity is seen in the liver, kidney and intestine.
Hereditary Fructose Intolerance
Rationale: Inability to convert fructose-1-phosphate and fructose- 1,6-bisphosphate into dihydroxyacetone phosphate, glyceraldehyde-3-phosphate and glyceraldehydes
Hereditary Fructose Intolerance
Clinical features: Irritability, lethargy, seizures and hepatomegaly
Hereditary Fructose Intolerance