ELECTRIPHOREISIS AND ELECTROCHEMISTRY
暂无描述。系统推荐的高质量记忆内容，适合每天坚持背诵学习。
卡片预览 (24 张)
→ A molecule that contains both acidic and basic groups
Ampholyte
The rate of migration of a charged solute in an electric field, expressed per unit field strength.
● Electrophoretic Mobility
Preferential movement of water in one direction through electrophoresis medium due to selective binding of one type of charge on the surface of the medium.
Endosmosis
→ The migration of charged macromolecules.
Electrophoretogram
The migration of small charge ions.
Iontophoresis
The migration of charged macromolecules.
Zone Electrophoresis
is acetylated to form cellulose acetate by treating it with acetic anhydride
Cellulose acetate
Different fractions of lipoproteins based on their density
High-density lipoproteins (HDL) ▪ Low-density lipoproteins (LDL) ▪ Intermediate-density lipoproteins (IDL) ▪ Very low-density lipoproteins (VLDL)
negatively charged
Cathode
positively charged
Anode
Versatile and powerful analytical technique capable of separating and analyzing a diverse range of ionized analytes.
ELECTROPHORESIS
Macromolecules of interest:
Proteins in serum → Urine → Cerebrospinal fluid (CSF) → Erythrocytes and tissue and, → Other biologic body fluids
Separates by electrical charge ● A highly purified uncharged polysaccharide derived from agar ● Neutral; therefore, doesn’t produce electroendosmosis
Agarose gel
CONVENTIONAL ELECTROPHORESIS (4)
→ Separation → Staining → Detection → Quantification
Referred to as PAGE Involves separation of protein on the basis of charge and molecular size ● Layers of gel with different pore sizes are used
Polyacrylamide gel
Used to separate macromolecules on the basis of both surface charge and molecular charge
Starch
Power supplies operating at either constant current or constant voltage are available commercially.
DRIVING FORCE (ELECTRICAL POWER)
is a molecule whose net charge can be either positive or negative
AMPHOLYTE
Serves as a multifunctional component in the electrophoretic process
BUFFER
Serum protein separation, Poor resolution, weak buffer
Barbitone buffer – (around 8.0 pH)
Enzyme separation, Low buffering capacity, high conductivity
Phosphate buffer – (around 7.0 pH)
Nucleic acid separation Good resolution, high buffering capacity, low conductivity
Tris – borate – EDTA buffer (TBE) – (pH around 8.0)
Nucleic acid separation High resolution, high buffering capacity, low conductivity
Tris – acetate – EDTA buffer (TAE) – (pH around 8.0)
Protein separation High buffering capacity, low conductivity
Tris – glycine buffer – (pH more than 8.0)