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LIPASE (LAB)

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Total cards: 9•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (9)

#1
Front (Question)

This substrate is cleaved by the pancreatic lipase

Back (Answer)

1,2-o-Dilauryl-rac-glycero-3-glutaric acid-(6’ methyl-resorufin) ester

#2
Front (Question)

The colorimetric substrate 1,2-o-Dilauryl-rac-glycero-3-glutaric acid-(6’ methyl-resorufin) ester is cleaved by pancreatic lipase and the resulting ____________________ is hydrolyzed under alkaline conditions to yield the _____________________.

Back (Answer)

dicarboxylic acid ester chromophore methylresorufin.

#3
Front (Question)

What is the volume of SERUM, CALIBRATOR, and BLANK in the LIPASE experiment?

Back (Answer)

20 uL

#4
Front (Question)

What is the volume of R1 in the LIPASE activity?

Back (Answer)

1000 uL

#5
Front (Question)

What is the volume of the R2 in the LIPASE ACTIVITY?

Back (Answer)

250 uL

#6
Front (Question)

What is the absorbance of lipase?

Back (Answer)

580nm

#7
Front (Question)

After all the absorbance has been averaged, what would be the next step?

Back (Answer)

Subtract Black from Serum to get the Final serum value and, Subract Blank from the Calibrator value to get the final calibrator value.

#8
Front (Question)

What is the formula of LIPASE?

Back (Answer)

Sample divided by the calibrator, multiplied to the calibrator value.

#9
Front (Question)

What is the normal value of LIPASE?

Back (Answer)

≤60 U/L