Bacteriology
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Number of bacterial morphology
4
Unit of measurement for bacteria
Micrometer (microns)
Unique component of the outer membrane of Gram-negative bacteria
Lipopolysaccharides
They grow in the absence of O2 and obtain O2 from O2 containing compounds
Anaerobes
Term used to denote “cold-loving” bacteria
Psychrophile / Cryophile
Nutrient media is made of extracts of _____ & _____
Meats & soybeans
Proper manner of dispensing culture media
Weigh agar Dissolve agar in flask Sterilize Dispense into petri dish
To dissolve the agar powder, pour half the amount of _____ in a vessel, then the _____ powder, then pour the rest of the _____
Water - agar - water
Type of water used in preparing culture media
Deionized / Distilled water
How to determine if an agar is completely dissolved?
Should be CLEAR (no particles)
Phenylethylalcohol is added on certain media to inhibit
Gram (-) bacteria
Why would thioglycollate broth be boiled for 10 minutes before being used?
To drive off oxygen
In thioglycollate broth, organisms will grow only in the part where _____ concentration meets their needs
Oxygen
__________ grow at the TOP of thioglycollate broth
Obligate aerobes
__________ grow NEAR THE TOP of thioglycollate broth
Microaerophiles
__________ grow at the BOTTOM of thioglycollate broth
Obligate anaerobes
__________ & __________ grow in the ENTIRE thioglycollate broth
Facultative anaerobes & Aerotolerant anaerobes
Added to the culture medium to tubes to provide and maintain an anaerobic environment
Mineral oil
Composition of blood agar
Nutrient agar + 5% sheep blood
How is chocolate agar prepared
Applying heat to blood agar to lyse RBC’s
Thayer-Martin medium is basically an __________ agar
Enriched chocolate agar
Clear medium for mycobacteria
Middlebrook 7H10 / 7H11
Component in SDA that is used as energy source
Dextrose
To make agar selective for fungi, what should be added on the medium?
Antibiotics + cycloheximide