Chromatography and Mass Spectrometry

Which of the following statements is TRUE? a. Partition chromatography is most appropriate for identifying analytes that may be distributed between two liquid phases. b. Steric exclusion chromatography is best suited for separating analytes based on their solubility in the mobile solvent. c. In liquid–solid chromatography, the stationary phase separates analytes based on size, shape, and polarity. d. Ion-exchange chromatography has a resin phase that is soluble to water, and separation of the mixture is based on magnitude and charge of ionic species. e. The partition coefficient is measured and compared with standards in thin-layer chromatography.

In high-performance thin-layer chromatography (HPTLC), developed bands are compared with reference standard concentrations. Each band is measured by: a. Mass spectrometer b. Densitometer c. Ruler d. Biuret protein assay e. Two-dimensional electrophoresis

In which of the following components of a chromatography instrument does selective separation of a mixture occur? a. Sample injection port b. Column c. Spectrometer d. Quadrupole e. Mass analyzer

True or False? In chromatography, the stationary phase is always of a solid matrix.

Mass spectrometry identifies analytes based on: a. Mass-to-charge ratio b. Retention factor c. Density of the band d. Molecular weight e. Solubility in the mobile phase

Drugs of abuse are typically measured by: a. Thin-layer chromatography b. Liquid–liquid chromatography c. Gas–liquid chromatography d. Steric exclusion chromatography e. HPLC

PCR/ESI-TOF has the distinct advantage in pathogen identification because: a. It requires the patient sample to be cultured and then analysis can be performed b. It can be used directly from patient specimens c. It uses the protein “fingerprint” to identify the pathogen.