Module 3 Translation, Elongation and Termination for E. Coli

Translation, Elongation and Termination for E. Coli: The 50S ribosomal subunit of E coli consists of 3 things: 1) that binds incoming tRNAs 2) binds charged tRNAs carrying amino acids that have formed peptide bonds with the growing polypeptide chain but not have dissociated. 3) releases dissociated tRNAs so they can be recharged with new amino acids.

tRNAs that can by-pass the A Aminoacyl site Eukaryotes: Prokaryotes: They both free up the A site to accept the tRNA after the start codon

Translation, Elongation and Termination for E. Coli: ___ proceeds with charged tRNAs entering the A site and then shifting to the P sites followed by the E site.

In every step of Elongation: The energy is provided by a ____ that ____ Through the ___ the ____ then form between the amino group of the amino acid attached to the A-site-tRNA and the _____ of the Amino acid attached to the P-site tRNA After the amino-acid in P-site is linked, it enters the E site which then the previous tRNA detaches from the amino acid

E.Coli Elongation: The _____ takes only ___ seconds to add each amino acid 200 amino acids can be added in 10 seconds

____ occurs when a ____ codon (UAA, UAG or UGA) is encountered. This is recognized by the ___. They then instruct ____ to add a water molecule to the ___ of the P-site amino acid which causes the detachment.

Summary: Initiation: Elongation: Termination:

Elongation factor purposes (3)

Large ribosomal unit contains: (3) Small ribosomal unit contains:

The elongation cycle of translation - overview • ____ An incoming aminoacyl tRNA binds to the codon in A site • ____ Ribosome catalyzes the formation of a peptide bond between the new amino acid and the carboxyl end of the growing polypeptide • ____ The tRNA in A site is translocated to the P site. Then the previous tRNA which was in the P site is moved to the E site where it is released.