Exam II Acid/Base Balance, Electrolytes, Osmometer
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Major intracellular cation Normal value
Potassium 3.5-5.0 mM/L
Major extracellular cation Normal Value
Sodium 136-145 mM/L
Major extracellular anion Normal Value
Chloride 90-109 mM/L
What is the endpoint of mercurimetric chloride assay?
when excess Hg2+ forms a complex with an indicator like dyphenylcarbazon, producing a violet-blue color
What two cations are most likely to be ordered to determine the cause of cardiac tetany?
calcium magnesium
calculate anion gap. be able to correlate abnormal anion gaps with other test results (such as BUN) or with instrument/tech errors
(Na + K)-(Cl + HCO3) = 12-20mmol/L Na - (Cl + HCO3) = 8-18 mmol/L
What does %saturation refer to? Normal value?
%saturation= serumFe/TIBC x 100 20-50%
What is hypokalemia?
low serum potassium caused by dietary intake
If tetany is due to calcium, what specific fraction of calcium is involved? Will it be increased or decreased?
Ionized(free) calcium decreased
What analyte in the blood is used to measure serum phosphorous? What is the normal value of serum phosphorous?
inorganic phosphorous 2.7-4.5 mg%
Why is it important to measure serum magnesium levels?
check for tetany due to magnesium deficiency
In analyzing calcium by atomic absorption, why is lanthanum added?
to prevent interference of phosphate
Calcium exist in serum as? Which is physiologically active?
ionized(free) and protein bound free
A patient with lactic acid acidosis would have an (increased/decreased) anion gap?
increased
What is the purpose of adding magnesium carbonate to an iron binding capacity?
to remove excess unbound iron
What does TIBC measure?
estimates transferrin level in serum
In what method is phosphate in serum combined with ammonium molybdate to form phosphomolybdate, which is then reduced to aminonapthosulfonic acid which is measured with spectrophotometery?
Fiske-Subbarow method for Phosphorus
Serum calcium and phosphorous have what kind of relationship?
reciprocal
What is the normal serum calcium level?
8.4-10.2 mg%
What would be the expected test results for calcium and phosphorus in hyperparathyroidism?
increase calcium decrease phosphorus
What is the purpose of adding stannous chloride, ferrous sulfate, and ascorbic acid in the determination of inorganic phosphorus?
reducing agents *reduces phosphomolybdate to aminonapthosulfonic acid
Name several things that can affect the level of circulating calcium
Vitamin D serum protein levels bone cell activity
What two hormones control serum calcium levels?
PTH and Calcitonin have an antagonistic relationship
Which electrolyte is the chief plasma base that helps in maintaining osmotic pressure?
sodium