Other Body Fluids
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A tracheobronchial secretion; a mixture of upper and lower respiratory tract
Sputum
Most preferred sample for routine sputum analysis
1st morning
Sputum collection method for unconscious / ventilated patients
Tracheal aspiration
Color of Charcot-Leyden crystals using Wright stain
Pink or purple
% population of lymphocytes in the broncheoalveolar lavage
1-15%
This cell in the broncheoalveolar lavage is elevated in cigarette smokers
Neutrophil
Sweat chloride of >70 mEq/L indicates
Cystic fibrosis
Puncture site when performing CSF lumbar puncture
Between 3rd and 4th / 4th and 5th lumbar vertebrae
Added to CSF to increase cell yield prior to cytocentrifugation
30% albumin
Diluent used for TOTAL CELL COUNT in the CSF
Normal saline
Diluent used for the CSF WBC COUNT
3% acetic acid with methylene blue
Subtract 1 WBC for every _____ RBCs seen in the CSF
700
Predominant normal cells in the CSF
Lymphocytes and monocytes
Normal value for CSF glucose
60-70% of plasma glucose
For an accurate evaluation of CSF glucose, a __________ must be run for comparison
Blood glucose test
Specimen for blood glucose should be drawn ___ hours prior to spinal tap to allow time for equilibrium
2
Normal value for CSF lactate
10-22 mg/dL
Normal value for CSF protein in adults
15-45 mg/dL
2nd most abundant CSF protein
Prealbumin
Most routinely used techniques for measuring total CSF protein
Turbidimetry and dye-binding
Concentration of TCA (&SSA) for CSF total protein determination
3%
IgG index indicative of immunoglobulin production within the CNS
> 0.70 (or >0.77)
Analyte used to identify unknown body fluid as CSF
Prealbumin / “tau” transferrin
In CSF electrophoresis by immunofixation or isoelectric focusing, what stain is used?
Silver stain