CC2 LAB: CREATININE KINASE, ALT, AST

associated with atp regeneration in contractile or transport system

ck is predominant in

how many kDa in ck

separation of atypical bands detection of ak

good with technique, may be more sensitive and precise

measure concentration rather than activity may detect inactive ck-mb

• anti-M inhibits M activity • CK activity is measure before and after inhibition • residual activity is multiplied by 2 to account for MB (50% inhibited) • disadvantage: detects BB activity; though small amount, may cause false MB activity; also, atypical forms are not inhibited

A in Coupled-enzyme method

forward reaction in ck

• reverse reaction in ck • most common • 2 – 6x faster than forward • less interference

Tanzer-Gilvarg Method pH

Oliver-Rosalki Method pH

inactivated because of oxidation of sulfhydryl groups

T/F: Hemolyzed samples falsely elevate CK activity

ratio of ck

working reagent (microliter) ck & ck-mb

sample (microliter) ck & ck-mb

Transfer of an amino group between aspartate and alpha- keto acids to form oxaloacetate and glutamate

coenzyme of ast

former name of ast

Consists of a coupled enzymatic reaction using Lactate Dehydrogenase as the indicator enzyme

Transfer of an amino group between alanine and alpha- keto acids to form glutamate and pyruvate

coenzyme of alt

former name of alt