Chapter 2 Concept Questions

Liquid is one of the ______ states which is used to classify microbial media.

Media that contain at least one ingredient that is not chemically definable are called ______ or nonsynthetic.

Media with a firmness midway between that of a broth (a liquid medium) and an ordinary solid medium are called ______ media.

Select all that apply Media ingredients that cannot be chemically defined include which of the following? ☐Blood and serum ☐Extracts of animals, plants, or yeasts ☐Sodium chloride ☐Disodium hydrogen phosphate ☐Cell secretions

The polysaccharide found in a sea alga that is commonly used to prepare solid culture media is called ______.

DNA detection technologies have shown that there are ______ microbes that we don’t know how to cultivate in the lab than those we do.

Select all that apply Which of the following attributes make agar an ideal solidifying agent for culturing media? Multiple select question. ☐It is solid at room temperature. ☐It is readily available but expensive. ☐It is a nutrient for many microbes. ☐It holds moisture and nutrients.

Select all that apply General purpose media ______. Multiple select question. ☐include nutrient agar and broth ☐usually contain a mixture of nutrients ☐grow a broad spectrum of microbes ☐include Mannitol salt and MacConkey agar ☐are usually non-synthetic ☐are usually chemically defined synthetic media

Which of the following media can be described precisely as to its chemical content? Multiple choice question. ○Defined ○General purpose ○Brain-Heart Infusion Broth ○Complex

Sabouraud’s agar restricts the growth of bacteria and is an example of a 1.[ ] medium, whereas Mannitol salt agar can determine which species of Staphylococcus is present, making it an example of a 2.[ ] medium.

Select all that apply Which types of media contain at least one ingredient that is not chemically defined? Multiple select question. ☐Complex ☐Chemically defined ☐Synthetic ☐Blood agar

Which term describes a discrete mound of cells growing on a solid nutrient surface? Multiple choice question. ○ petri dish ○ assay medium ○ mixed culture ○ colony

All of the following are media ingredients that cannot be chemically defined EXCEPT ○ serum ○ blood ○ meat extracts or infusions ○ sodium chloride

“Inspection” begins the process using the Five “I” procedure for culturing microorganisms. ○True ○False

Given the vast diversity of microbes in nature, _____ microbes can be cultured on artificial medium. Multiple choice question. ○most ○all ○some

Which type(s) of organisms are usually observed with light microscopes? Multiple choice question. ○Prions ○Arthropods ○Bacteria ○Viruses

General purpose media are characterized by ______. Multiple choice question. ○allowing the growth of a broad range of microbes ○containing only one nonsynthetic nutrient ○being synthetic and chemically definable

Selective media cannot be differential. ○True ○False

For a light microscope, the lens that is normally located directly above the stage is called the ______ lens.

When adequately separated from other cells, an individual bacterial cell can grow into a discrete mound of cells called a(n)

Select all that apply The total power of magnification of the final image is a product of the separate powers of which two microscope components? Multiple select question. ☐objective lens ☐aperture ☐diaphragm ☐condenser ☐ocular lens

[ ] is part of the Five I’s of culturing microorganisms and is based on visual observations of microscopic and macroscopic growth characteristics.

This image of a Paramecium was obtained using _____ microscopy. ○bright-field ○dark-field ○fluorescence ○scanning electron

The ______ microscope is used to visualize most types of bacteria. ○electron ○light ○atomic force