Analytical Techniques and Automation
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Describes the measurement principles used in the clinical chemistry laboratory
Analytic Techniques
Examples of analytic techniques
Spectrophotometry Electrochemistry Electrophoresis Chromatography
Analytic techniques often used to determine concentrations of analytes in the CC lab
Spectrophotometry Electrochemistry
Described as photons of energy traveling in waves
Electromagnetic radiation
Most recognizable forms of electromagnetic radiation
Light and radiant energy
Other types of electromagnetic radiation
Gamma rays X-rays Microwaves Ultraviolet radiation Radiofrequency Radiation
The linear distance between any two equivalent points on a successive wave
Wavelength
Unit used in the visible spectrum
Nanometer nm
The relationship between wavelength and energy (E) is described by
Planck’s formula
Planck’s formula
𝐸 = h𝑣
Planck’s constant value
6.62 x10^-27 erg/sec
Unit of energy in the centimeter gram second system unit
erg
Is erg an SI unit?
No
h in the Planck’s formula
Planck’s constant
v in Planck’s in formula
Frequency
Number of oscillations of the waveform in a second
Frequency
Changes that occur in a period of time. Movement of waveform
Oscillation
Relationship between wavelength and frequency
Inversely proportional
Relationship between energy of electromagnetic radiation and wavelength
Inversely proportional
Wavelength of visible region
400-700nm
Wavelength of ultraviolet region
<400nm
Wavelength of infrared region
> 700nm
Visible region falls in between
Color violet (set at 400nm) and red (at 700nm)
States that the concentration of a substance is directly proportional to the amount of light absorbed or inversely proportional to the algorithm of the transmitted light
Beer’s Law